Jeong, Daun Yang, Jeongmo Lee, Soojin Kim, Borim Um, Youngsoon Kim, Youngrok Ha, Kyoung-Su Lee, Jinwon 2024-01-20T04:02:33Z 2024-01-20T04:02:33Z 2022-01-25 2016-06 1082-6068 https://pubs.kist.re.kr/handle/201004/123982 Klebsiella pneumoniae is known to produce 2,3-butanediol (2,3-BDO), a valuable chemical. In K. pneumoniae, the 2,3-BDO operon (budBAC) is involved in the production of 2,3-BDO. To observe the physiological role of the 2,3-BDO operon in a mixed acid fermentation, we constructed a budBAC-deleted strain (SGSB109). The production of extracellular metabolites, CO2 emission, carbon distribution, and NADH/NAD(+) balance of SGSB109 were compared with the parent strain (SGSB100). When comparing the carbon distribution at 15hr, four significant differences were observed: in 2,3-BDO biosynthesis, lactate and acetate production (lactate and acetate production increased 2.3-fold and 4.1-fold in SGSB109 compared to SGSB100), CO2 emission (higher in SGSB100), and carbon substrate uptake (higher in SGSB100). Previous studies on the inactivation of the 2,3-BDO operon were focused on the increase of 1,3-propanediol production. Few studies have been done observing the role of 2,3-BDO biosynthesis. This study provides a prime insight into the role of 2,3-BDO biosynthesis of K. pneumoniae. English Marcel Dekker Inc. Deletion of the budBAC operon in Klebsiella pneumoniae to understand the physiological role of 2,3-butanediol biosynthesis Article 10.1080/10826068.2015.1045603 1 Preparative Biochemistry and Biotechnology, v.46, no.4, pp.410 - 419 Preparative Biochemistry and Biotechnology 46 4 410 419 N scie scopus 000377930000014 2-s2.0-84975873886 Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article FERMENTATION STRAIN 2,3-butanediol budBAC operon carbon distribution analysis CO2 emission redox balance