Shafiq, Muhammad Jung, Youngmee Kim, Soo Hyun 2024-01-20T04:03:10Z 2024-01-20T04:03:10Z 2021-09-04 2016-06 1549-3296 https://pubs.kist.re.kr/handle/201004/124016 The development of cell-free vascular grafts has tremendous potential for tissue engineering. However, thrombus formation, less-than-ideal cell infiltration, and a lack of growth potential limit the application of electrospun scaffolds for in situ tissue-engineered vasculature. To overcome these challenges, here we present development of an acellular tissue-engineered vessel based on electrospun poly(L-lactide-co--caprolactone) scaffolds. Heparin was conjugated to suppress thrombogenic responses, and substance P (SP) was immobilized to recruit host cells. SP was released in a sustained manner from scaffolds and recruited human bone marrow-derived mesenchymal stem cells. The biocompatibility and biological performance of the grafts were evaluated by in vivo experiments involving subcutaneous scaffold implantation in Sprague-Dawley rats (n=12) for up to 4 weeks. Histological analysis revealed a higher extent of accumulative host cell infiltration, neotissue formation, collagen deposition, and elastin deposition in scaffolds containing either SP or heparin/SP than in the control groups. We also observed the presence of a large number of laminin-positive blood vessels, von Willebrand factor (vWF(+)) cells, and alpha smooth muscle actin-positive cells in the explants containing SP and heparin/SP. Additionally, SP and heparin/SP grafts showed the existence of CD90(+) and CD105(+) MSCs and induced a large number of M2 macrophages to infiltrate the graft wall compared with that observed with the control group. Our cell-free grafts could enhance vascular regeneration by endogenous cell recruitment and by mediating macrophage polarization into the M2 phenotype, suggesting that these constructs may be a promising cell-free graft candidate and are worthy of further in vivo evaluation. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1352-1371, 2016. English WILEY NEUROPEPTIDE SUBSTANCE-P BONE MORPHOGENETIC PROTEIN-2 PEPTIDE NANOFIBERS BLOOD-VESSELS RECRUITMENT ANGIOGENESIS SCAFFOLDS GROWTH POLY(L-LACTIDE-CO-EPSILON-CAPROLACTONE) INFILTRATION Covalent immobilization of stem cell inducing/recruiting factor and heparin on cell-free small-diameter vascular graft for accelerated in situ tissue regeneration Article 10.1002/jbm.a.35666 1 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v.104, no.6, pp.1352 - 1371 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 104 6 1352 1371 scie scopus 000375117200004 2-s2.0-84977858260 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article NEUROPEPTIDE SUBSTANCE-P BONE MORPHOGENETIC PROTEIN-2 PEPTIDE NANOFIBERS BLOOD-VESSELS RECRUITMENT ANGIOGENESIS SCAFFOLDS GROWTH POLY(L-LACTIDE-CO-EPSILON-CAPROLACTONE) INFILTRATION stem cell vascular graft electrospinning in situ tissue regeneration neovascularization/angiogenesis