Shin, Jihye Kim, Gamin Lee, Jong Won Lee, Ji Eun Kim, Yoo Seok Yu, Jong-Han Lee, Seung-Taek Ahn, Sei Hyun Kim, Hoguen Lee, Cheolju 2024-01-20T04:03:14Z 2024-01-20T04:03:14Z 2021-09-04 2016-06 1347-9032 https://pubs.kist.re.kr/handle/201004/124020 Cancer cell secretomes are considered a potential source for the discovery of cancer markers. In this study, the secretomes of four breast cancer (BC) cell lines (Hs578T, MCF-7, MDA-MB-231, and SK-BR-3) were profiled with liquid chromatography-tandem mass spectrometry analysis. A total of 1410 proteins were identified with less than 1% false discovery rate, of which approximately 55% (796 proteins) were predicted to be secreted from cells. To find BC-specific proteins among the secreted proteins, data of immunohistochemical staining compiled in the Human Protein Atlas were investigated by comparing the data of BC tissues with those of normal tissues. By applying various criteria, including higher expression level in BC tissues, higher predicted potential of secretion, and sufficient number of tandem mass spectra, 12 biomarker candidate proteins including ganglioside GM2 activator (GM2A) were selected for confirmation. Western blot analysis and ELISA for plasma samples of healthy controls and BC patients revealed elevation of GM2A in BC patients, especially those who were estrogen receptor-negative. Additionally, siRNA-mediated knockdown of GM2A in BC cells decreased migration in vitro, whereas the overexpression of GM2A led to an increase in cell migration. Although GM2A as a diagnostic and prognostic marker in BC should be carefully verified further, this study has established the potential role of GM2A in BC progression. English WILEY-BLACKWELL HUMAN PROTEIN ATLAS SEROLOGICAL BIOMARKERS PLASMA-PROTEOME IN-VITRO REVEALS COLLECTION DISCOVERY PATHWAYS MOTILITY PLATFORM Identification of ganglioside GM2 activator playing a role in cancer cell migration through proteomic analysis of breast cancer secretomes Article 10.1111/cas.12935 1 CANCER SCIENCE, v.107, no.6, pp.828 - 835 CANCER SCIENCE 107 6 828 835 scie scopus 000378715100014 2-s2.0-84979084049 Oncology Oncology Article HUMAN PROTEIN ATLAS SEROLOGICAL BIOMARKERS PLASMA-PROTEOME IN-VITRO REVEALS COLLECTION DISCOVERY PATHWAYS MOTILITY PLATFORM Biomarker breast cancer ganglioside GM2 activator migration secretome