Choi, Sojoong Kim, Hyunju Kim, So Yeon Yang, Eun Gyeong 2024-01-20T04:03:23Z 2024-01-20T04:03:23Z 2021-09-03 2016-06 2040-3364 https://pubs.kist.re.kr/handle/201004/124029 Most proteins perform their functions as interacting complexes. Here we propose a novel method for capturing an intracellular protein and its interacting partner out of living cells by utilizing intracellular access of antibody modified vertical silicon nanowire arrays whose surface is covered with a polyethylene glycol layer to prevent strong cell adhesion. Such a feature facilitates the removal of cells by simple washing, enabling subsequent detection of a pulled-down protein and its interacting partner, and further assessment of a drug-induced change in the interacting complex. Our new SiNW-based tool is thus suitable for authentication of protein networks inside living cells. English ROYAL SOC CHEMISTRY BCR-ABL ENZYMATIC-ACTIVITY K562 CELLS PENETRATION INTERROGATION NANONEEDLES LEUKEMIA PLATFORM BINDS Probing protein complexes inside living cells using a silicon nanowire-based pull-down assay Article 10.1039/c6nr00171h 1 NANOSCALE, v.8, no.22, pp.11380 - 11384 NANOSCALE 8 22 11380 11384 scie scopus 000377919800005 2-s2.0-84973523419 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Chemistry Science & Technology - Other Topics Materials Science Physics Article BCR-ABL ENZYMATIC-ACTIVITY K562 CELLS PENETRATION INTERROGATION NANONEEDLES LEUKEMIA PLATFORM BINDS