Choi, Dong Hoon Kang, Sung Nam Kim, Seong Min Gobaa, Samy Park, Bang Ju Kim, Ik Hwan Joung, Yoon Ki Han, Dong Keun 2024-01-20T04:04:28Z 2024-01-20T04:04:28Z 2021-09-05 2016-05-01 0927-7765 https://pubs.kist.re.kr/handle/201004/124082 Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethy1-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER INTIMAL HYPERPLASIA ELUTING STENTS PROTEIN IMMOBILIZATION BIOACTIVITY EXPRESSION SURFACES DELIVERY CELLS MODEL Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization Article 10.1016/j.colsurfb.2016.01.028 1 COLLOIDS AND SURFACES B-BIOINTERFACES, v.141, pp.602 - 610 COLLOIDS AND SURFACES B-BIOINTERFACES 141 602 610 scie scopus 000374197700071 2-s2.0-84959148135 Biophysics Chemistry, Physical Materials Science, Biomaterials Biophysics Chemistry Materials Science Article INTIMAL HYPERPLASIA ELUTING STENTS PROTEIN IMMOBILIZATION BIOACTIVITY EXPRESSION SURFACES DELIVERY CELLS MODEL Re-endothelialization Stent Hyaluronic acid Heparin Growth factor Dopamine