Lee, Seung Woo Lee, Eun-Hee Thiel, Gerhard Van Etten, James L. Saraf, Ravi F. 2024-01-20T04:04:36Z 2024-01-20T04:04:36Z 2022-01-25 2016-05 1936-0851 https://pubs.kist.re.kr/handle/201004/124090 Chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) contains a viral-encoded K+ channel imbedded in its internal membrane, which triggers host plasma membrane depolarization during virus infection. This early stage of infection was monitored at high resolution by recording the cell membrane depolarization of a single Chlorella cell during infection by a single PBCV-1 particle. The measurement was achieved by depositing the cells onto a network of one-dimensional necklaces of Au nanoparticles, which spanned two electrodes 70 mu m apart. The nanoparticle necklace array has been shown to behave as a single-electron device at room temperature. The resulting electrochemical field-effect transistor (eFET) was gated by the cell membrane potential, which allowed a quantitative measurement of the electrophysiological changes across the rigid cell wall of the microalgae due to a single viral attack at high sensitivity. The single viral infection signature was quantitatively confirmed by coupling the eFET measurement with a method in which a single viral particle was delivered for infection by a scanning probe microscope cantilever. English AMER CHEMICAL SOC Noninvasive Measurement of Electrical Events Associated with a Single Chlorovirus Infection of a Microalgal Cell Article 10.1021/acsnano.6b00299 1 ACS NANO, v.10, no.5, pp.5123 - 5130 ACS NANO 10 5 5123 5130 N scie scopus 000376825100024 2-s2.0-84973369581 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article FIELD-EFFECT TRANSISTOR CHLORELLA-LIKE ALGAE ROOM-TEMPERATURE ION CHANNELS VIRUS NANOPARTICLES PBCV-1 MICROORGANISMS TRANSPORT NETWORK field-effect transistor viral infection cell membrane potential depolarization electrochemical analysis