Yeom, Sang Yun Son, Choong Hyun Kim, Byung Sun Tag, Sung Hyun Nam, Eunjoo Shin, Hyogeun Kim, So Hyun Gang, Haemin Lee, Hyunjoo J. Choi, Jungkyu Im, Heh-In Cho, Il-Joo Choi, Nakwon 2024-01-20T04:31:29Z 2024-01-20T04:31:29Z 2021-09-05 2016-04-19 0003-2700 https://pubs.kist.re.kr/handle/201004/124163 Epigenetic alterations in gene expression are influenced by experiences and environment, resulting in significant variation of epigenetic markers from individual to individual. Therefore, it is imperative to measure various epigenetic markers simultaneously from samples of individual subjects to accurately analyze the epigenetic markers in biological samples. Moreover, the individualized genome-wide analysis has become a critical technology for recent trends in clinical applications such as early diagnosis and personalized medicine screening of numerous diseases. The array-based detection of modified histones, conventionally used for multiplexed analysis of epigenetic changes, requires pooling of samples from many subjects to analyze population wise differences in the expression of histone markers and does not permit individualized analysis. Here, we report multiplexed detection of genome-wide changes in various histone modifications at a single-residue resolution using quantum dot (QP)-encoded polyethylene glycol diacrylate (PEGDA) hydrogel microparticles. To demonstrate the potential of our methodology, we present the simultaneous detection of (I) acetylation of lysine 9 of histone 3 (Ac-H3K9), dimethylation of H3K9 (2Me-H3K9), and (3) trimethylation of H3K9 (3Me-H3K9) from three distinct regions in the brain [nucleus accumbens (NAc), dorsal striatum (DSt), and cerebellum (Cbl)] of cocaine-exposed mice. Our hydroger-based epigenetic assay enabled relative quantification of the three histone variants from only 10 mu L of each brain lysate (protein content = similar to 1 mu g/mu L) per mouse. We verified that the exposure to cocaine induced a significant increase of acetylation while a notable decrease in methylation in NAc. English AMER CHEMICAL SOC COCAINE-INDUCED PLASTICITY NUCLEUS-ACCUMBENS HISTONE MODIFICATION PROTEIN-DETECTION DRUG-ADDICTION PARTICLES CHROMATIN QUANTIFICATION ACETYLATION METHYLATION Multiplexed Detection of Epigenetic Markers Using Quantum Dot (QD)-Encoded Hydrogel Microparticles Article 10.1021/acs.analchem.5b04190 1 ANALYTICAL CHEMISTRY, v.88, no.8, pp.4259 - 4268 ANALYTICAL CHEMISTRY 88 8 4259 4268 scie scopus 000374706000016 2-s2.0-84966351177 Chemistry, Analytical Chemistry Article COCAINE-INDUCED PLASTICITY NUCLEUS-ACCUMBENS HISTONE MODIFICATION PROTEIN-DETECTION DRUG-ADDICTION PARTICLES CHROMATIN QUANTIFICATION ACETYLATION METHYLATION