Yoon, Jeong-Kee Kim, Hong Nam Bhang, Suk Ho Shin, Jung-Youn Han, Jin La, Wan-Geun Jeong, Gun-Jae Kang, Seokyung Lee, Ju-Ro Oh, Jaesur Kim, Min Sung Jeon, Noo Li Kim, Byung-Soo 2024-01-20T04:32:20Z 2024-01-20T04:32:20Z 2021-09-05 2016-04 1937-3341 https://pubs.kist.re.kr/handle/201004/124208 The rapid recruitment of osteoblasts in bone defects is an essential prerequisite for efficient bone repair. Conventionally, osteoblast recruitment to bone defects and subsequent bone repair has been achieved using growth factors. Here, we present a methodology that can guide the recruitment of osteoblasts to bone defects with topographically defined implants (TIs) for efficient in vivo bone repair. We compared circular TIs that had microgrooves in parallel or radial arrangements with nonpatterned implants for osteoblast migration and in vivo bone formation. In vitro, the microgrooves in the TIs enhanced both the migration and proliferation of osteoblasts. Especially, the microgrooves with radial arrangement demonstrated a much higher efficiency of osteoblast recruitment to the implants than did the other types of implants, which may be due to the efficient guidance of cell migration toward the cell-free area of the implants. The expression of the intracellular signaling molecules responsible for the cell migration was also upregulated in osteoblasts on the microgrooved TIs. In vivo, the TI with radially defined topography demonstrated much greater bone repair in mouse calvarial defect models than in the other types of implants. Taken together, these results indicate that implants with physical guidance can enhance tissue repair by rapid cell recruitment. English MARY ANN LIEBERT, INC GROWTH-FACTOR CELL MOTILITY E-CADHERIN SUBSTRATE TOPOGRAPHY MIGRATION STIFFNESS PROLIFERATION BIOMATERIALS CDC42 SPEED Enhanced Bone Repair by Guided Osteoblast Recruitment Using Topographically Defined Implant Article 10.1089/ten.tea.2015.0417 1 TISSUE ENGINEERING PART A, v.22, no.7-8, pp.654 - 664 TISSUE ENGINEERING PART A 22 7-8 654 664 scie scopus 000374761600008 2-s2.0-84966283942 Cell & Tissue Engineering Cell Biology Engineering, Biomedical Materials Science, Biomaterials Cell Biology Engineering Materials Science Article GROWTH-FACTOR CELL MOTILITY E-CADHERIN SUBSTRATE TOPOGRAPHY MIGRATION STIFFNESS PROLIFERATION BIOMATERIALS CDC42 SPEED