Park, In Soo Kwak, Tae Joon Lee, Gyudo Son, Myeonggu Choi, Jeong Woo Choi, Seungyeop Nam, Kihwan Lee, Sei-Young Chang, Woo-Jin Eom, Kilho Yoon, Dae Sung Lee, Sangyoup Bashir, Rashid Lee, Sang Woo 2024-01-20T04:32:27Z 2024-01-20T04:32:27Z 2021-09-05 2016-04 1936-0851 https://pubs.kist.re.kr/handle/201004/124215 The direct quantification of weak intermolecular binding interactions is very important for many applications in biology and medicine. Techniques that can be used to investigate such interactions under a controlled environment, while varying different parameters such as loading rate, pulling direction, rupture event measurements, and the use of different functionalized probes, are still lacking. Herein, we demonstrate a biaxial dielectrophoresis force spectroscopy (BDFS) method that can be used to investigate weak unbinding events in a high-throughput manner under controlled environments and by varying the pulling direction (i.e., transverse and/or vertical axes) as well as the loading rate. With the BDFS system, we can quantitatively analyze binding interactions related to hydrogen bonding or ionic attractions between functionalized microbeads and a surface within a microfluidic device. Our BDFS system allowed for the characterization of the number of bonds involved in an interaction, bond affinity, kinetic rates, and energy barrier heights and widths from different regimes of the energy landscape. English AMER CHEMICAL SOC SINGLE-MOLECULE ADHESION PROTEIN MICROSCOPY RESOLUTION TWEEZERS AFM Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions Article 10.1021/acsnano.5b05286 1 ACS NANO, v.10, no.4, pp.4011 - 4019 ACS NANO 10 4 4011 4019 scie scopus 000375245000015 2-s2.0-84968764208 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article SINGLE-MOLECULE ADHESION PROTEIN MICROSCOPY RESOLUTION TWEEZERS AFM dielectrophoresis force spectroscopy intermolecular weak binding interactions energy landscape microfluidic device pulling direction