Seo, Young Hun Singh, Ajay Cho, Hong-Jun Kim, Youngsun Heo, Jeongyun Lim, Chang-Keun Park, Soo Young Jang, Woo-Dong Kim, Sehoon 2024-01-20T04:32:35Z 2024-01-20T04:32:35Z 2021-09-05 2016-04 0142-9612 https://pubs.kist.re.kr/handle/201004/124223 H2O2-Specific peroxalate chemiluminescence is recognized as a potential signal for sensitive in vivo imaging of inflammation but the effect of underlying peroxalate-emitter energetics on its efficiency has rarely been understood. Here we report a simple nanophotonic way of boosting near-infrared chemiluminescence with no need of complicated structural design and synthesis of an energetically favored emitter. The signal enhancement was attained from the construction of a nanoparticle imaging probe (similar to 26 nm in size) by dense nanointegration of multiple molecules possessing unique photonic features, i.e., i) a peroxalate as a chemical fuel generating electronic excitation energy in response to inflammatory H2O2, ii) a low-bandgap conjugated polymer as a bright near-infrared emitter showing aggregation induced emission (AIE), and iii) an energy gap-bridging photonic molecule that relays the chemically generated excitation energy to the emitter for its efficient excitation. From static and kinetic spectroscopic studies, a green-emissive BODIPY dye has proven to be an efficient relay molecule to bridge the energy gap between the AIE polymer and the chemically generated excited intermediate of H2O2-reacted peroxalates. The energy-relayed nanointegration of AIE polymer and peroxalate in water showed a 50-times boosted sensing signal compared to their dissolved mixture in THF. Besides the high H2O2 detectability down to 10(-9) M, the boosted chemiluminescence presented a fairly high tissue penetration depth (>12 mm) in an ex vivo condition, which enabled deep imaging of inflammatory H2O2 in a hair-covered mouse model of peritonitis. (C) 2016 Elsevier Ltd. All rights reserved. English ELSEVIER SCI LTD HYDROGEN-PEROXIDE NANOPARTICLES EMISSION FLUORESCENCE NANOPROBES PROBES RED Rational design for enhancing inflammation-responsive in vivo chemiluminescence via nanophotonic energy relay to near-infrared AIE-active conjugated polymer Article 10.1016/j.biomaterials.2016.01.038 1 BIOMATERIALS, v.84, pp.111 - 118 BIOMATERIALS 84 111 118 scie scopus 000371367500010 2-s2.0-84978835916 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article HYDROGEN-PEROXIDE NANOPARTICLES EMISSION FLUORESCENCE NANOPROBES PROBES RED Hydrogen peroxide Chemiluminescence Inflammation AIE-active conjugated polymer Nanoparticles