Kang, Jung-Woo Shin, Jun-Kyu Koh, Eun-Ji Ryu, Hyojeong Kim, Hyoung Ja Lee, Sun-Mee 2024-01-20T04:32:39Z 2024-01-20T04:32:39Z 2021-09-05 2016-04 0271-5317 https://pubs.kist.re.kr/handle/201004/124227 Opuntia ficus-indica (L.) is a popular edible plant that possesses considerable nutritional value and exhibits diverse biological actions including anti-inflammatory and antidiabetic activities. In this study, we hypothesized that DWJ504, an extract of O ficus-indica seed, would ameliorate hepatic steatosis and inflammation by regulating hepatic de novo lipogenesis and macrophage polarization against experimental nonalcoholic steatohepatitis. Mice were fed a normal diet or a high-fat diet (HFD) for 10 weeks. DWJ504 (250, 500, and 1000 mg/kg) or vehicle (0.5% carboxymethyl cellulose) were orally administered for the last 4 weeks of the 10-week HFD feeding period. DWJ504 treatment remarkably attenuated HFD-induced increases in hepatic lipid content and hepatocellular damage. DWJ504 attenuated increases in sterol regulatory element-binding protein 1 and carbohydrate-responsive element-binding protein expression and a decrease in camitine palmitoyltransferase 1A. Although DWJ504 augmented peroxisome proliferator activated receptor a protein expression, it attenuated peroxisome proliferator activated receptor gamma expression. Moreover, DWJ504 promoted hepatic M2 macrophage polarization as indicated by attenuation of the M1 marker genes and enhancement of M2 marker genes. Finally, DWJ504 attenuated expression of toll-like receptor 4, nuclear factor kappa B, tumor necrosis factor alpha, interleukin 6, TIR-domain-containing adapter inducing interferon beta, and interferon beta levels. Our results demonstrate that DWJ504 prevented intrahepatic lipid accumulation, induced M2 macrophage polarization, and suppressed the toll-like receptor 4-mediated inflammatory signaling pathway. Thus, DWJ504 has therapeutic potential in the prevention of nonalcoholic fatty liver disease. (C) 2016 Elsevier Inc. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD TOLL-LIKE RECEPTORS LIVER-DISEASE OXIDATIVE STRESS NONALCOHOLIC STEATOHEPATITIS KUPFFER CELLS VAR. SABOTEN ACTIVATION CACTUS RATS OBESITY Opuntia ficus-indica seed attenuates hepatic steatosis and promotes M2 macrophage polarization in high-fat diet-fed mice Article 10.1016/j.nutres.2015.12.007 1 NUTRITION RESEARCH, v.36, no.4, pp.369 - 379 NUTRITION RESEARCH 36 4 369 379 scie scopus 000373247100009 2-s2.0-84960100690 Nutrition & Dietetics Nutrition & Dietetics Article TOLL-LIKE RECEPTORS LIVER-DISEASE OXIDATIVE STRESS NONALCOHOLIC STEATOHEPATITIS KUPFFER CELLS VAR. SABOTEN ACTIVATION CACTUS RATS OBESITY High-fat diet fed mice Lipid accumulation Macrophage polarization Non-alcoholic fatty liver disease Opuntia ficus-indica seed Toll-like receptor 4