Park, J. Y. Shin, M. S. Kim, S. N. Kim, H. Y. Kim, K. H. Shin, K. S. Kang, K. S. 2024-01-20T04:32:43Z 2024-01-20T04:32:43Z 2021-09-05 2016-04 0141-8130 https://pubs.kist.re.kr/handle/201004/124230 Although the peel of the hallabong (Citrus sphaerocarpa) fruit is rich in polysaccharides, which are valuable dietary ingredients for human health, it is normally wasted. The present study aimed to utilize the peel waste and identify properties it may have against breast cancer metastasis. Hallabong peel extract containing crude polysaccharides was fractionated by gel permeation chromatography to produce four different polysaccharide fractions (HBE-I, -II, -III, and -IV). The HBE polysaccharides significantly blocked tube formation of human umbilical vein vascular endothelial cells (HUVECs), at a concentration of 12.5 or 25 mu g/mL. Tube formation appeared to be more sensitive to HBE-II than to other HBE polysaccharides. HBE-II also inhibited breast cancer cell migration, through downregulation of matrix metalloproteinase9 (MMP-9) in MDA-MB-231 triple-negative breast cancer cells. Therefore, inhibition of tube formation and MMP-9-mediated migration observed in HUVEC and MDA-MB-231 cells, respectively, are likely to be important therapeutic targets in triple-negative breast cancer metastasis. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV MATRIX METALLOPROTEINASES METASTASIS GROWTH POLYMETHOXYFLAVONES STRATEGIES INVASION THERAPY WALLS ACID Polysaccharides from Korean Citrus hallabong peels inhibit angiogenesis and breast cancer cell migration Article 10.1016/j.ijbiomac.2016.01.015 1 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.85, pp.522 - 529 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 85 522 529 scie scopus 000370886800061 2-s2.0-84954348680 Biochemistry & Molecular Biology Chemistry, Applied Polymer Science Biochemistry & Molecular Biology Chemistry Polymer Science Article MATRIX METALLOPROTEINASES METASTASIS GROWTH POLYMETHOXYFLAVONES STRATEGIES INVASION THERAPY WALLS ACID Hallabong Polysaccharide Angiogenesis Breast cancer Matrix metalloproteinase-9