Yoon, Hwa In Yhee, Ji Young Na, Jin Hee Lee, Sangmin Lee, Hyukjin Kang, Sun-Woong Chang, Hyeyoun Ryu, Ju Hee Lee, Seulki Kwon, Ick Chan Cho, Yong Woo Kim, Kwangmeyung 2024-01-20T04:32:44Z 2024-01-20T04:32:44Z 2021-09-05 2016-04 1043-1802 https://pubs.kist.re.kr/handle/201004/124231 Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) to generate unnatural azide groups (-N-3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 x 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo. English AMER CHEMICAL SOC STEM-CELLS STRATEGIES THERAPY PROLIFERATION NANOPARTICLES SELECTIVITY CARTILAGE SURVIVAL DISEASE Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo Article 10.1021/acs.bioconjchem.6b00010 1 BIOCONJUGATE CHEMISTRY, v.27, no.4, pp.927 - 936 BIOCONJUGATE CHEMISTRY 27 4 927 936 scie scopus 000374812600011 2-s2.0-84965082415 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry, Organic Biochemistry & Molecular Biology Chemistry Article STEM-CELLS STRATEGIES THERAPY PROLIFERATION NANOPARTICLES SELECTIVITY CARTILAGE SURVIVAL DISEASE Bioorthogonal Click Chemistry DBCO-650 Cell therapy Chondrocytes