Lee, Jaekwang Han, Young-Eun Favorov, Oleg Tommerdahl, Mark Whitsel, Barry Lee, C. Justin 2024-01-20T04:32:51Z 2024-01-20T04:32:51Z 2021-09-04 2016-04 1226-2560 https://pubs.kist.re.kr/handle/201004/124238 Regulation of cell volume is an important aspect of cellular homeostasis during neural activity. This volume regulation is thought to be mediated by activation of specific transporters, aquaporin, and volume regulated anion channels (VRAC). In cultured astrocytes, it was reported that swelling-induced mitogen-activated protein (MAP) kinase activation is required to open VRAC, which are thought to be important in regulatory volume decrease and in the response of CNS to trauma and excitotoxicity. It has been also described that sodium fluoride (NaF), a recognized G-protein activator and protein phosphatase inhibitor, leads to a significant MAP kinase activation in endothelial cells. However, NaF's effect in volume regulation in the brain is not known yet. Here, we investigated the mechanism of NaF-induced volume change in rat and mouse hippocampal slices using intrinsic optical signal (IOS) recording, in which we measured relative changes in intracellular and extracellular volume as changes in light transmittance through brain slices. We found that NaF (1 similar to 5 mM) application induced a reduction in light transmittance (decreased volume) in CA1 hippocampus, which was completely reversed by MAP kinase inhibitor U0126 (10 mu M). We also observed that NaF-induced volume reduction was blocked by anion channel blockers, suggesting that NaF-induced volume reduction could be mediated by VRAC. Overall, our results propose a novel molecular mechanism of NaF-induced volume reduction via MAP kinase signaling pathway by activation of VRAC. English KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE INTRINSIC OPTICAL SIGNALS MITOGEN-ACTIVATED PROTEIN SODIUM-FLUORIDE EXTRACELLULAR-SPACE EXPRESSION RELEASE STRESS NEUROTOXICITY IMPAIRMENT ASTROCYTES Fluoride Induces a Volume Reduction in CA1 Hippocampal Slices Via MAP Kinase Pathway Through Volume Regulated Anion Channels Article 10.5607/en.2016.25.2.72 1 EXPERIMENTAL NEUROBIOLOGY, v.25, no.2, pp.72 - 78 EXPERIMENTAL NEUROBIOLOGY 25 2 72 78 scopus kci ART002104264 000406858300003 2-s2.0-84995575848 Medicine, Research & Experimental Neurosciences Research & Experimental Medicine Neurosciences & Neurology Article INTRINSIC OPTICAL SIGNALS MITOGEN-ACTIVATED PROTEIN SODIUM-FLUORIDE EXTRACELLULAR-SPACE EXPRESSION RELEASE STRESS NEUROTOXICITY IMPAIRMENT ASTROCYTES Sodium Fluoride Hippocampus Volume regulation VRAC