Seo, Ji-Eun Hasan, Mahbub Han, Joon-Seung Kim, Nak-Kyoon Lee, Ji Eun Lee, Kang Mi Park, Ju-Hyung Kim, Ho Jun Son, Junghyun Lee, Jaeick Kwon, Oh-Seung 2024-01-20T04:32:52Z 2024-01-20T04:32:52Z 2021-09-04 2016-04 0364-3190 https://pubs.kist.re.kr/handle/201004/124239 Experimental autoimmune encephalomyelitis (EAE) is commonly induced with myelin oligodendrocyte glycoprotein (MOG)(35-55); occasionally, EAE is not well induced despite MOG(35-55) immunization. To confirm that EAE induction varies with difference in MOG(35-55) properties, we compared three MOG(35-55) from different commercial sources, which are MOG-A, MOG-B, and MOG-C. The peptides induced EAE disease with 100, 40, and 20 % incidence, respectively. Compared with others, MOG-A showed higher peptide purity (99.2 %) and content (92.2 %) and presented a sheet shape with additional sodium and chloride chemical elements. In MOG-A-treated group, MMP-9 activity and IL-6 levels were considerably higher than the other groups in CNS tissues, and significantly increased VCAM-1, IFN-gamma, and decreased IL-4 were also shown compared to MOG-B- and/or MOG-C-treated group. In conclusion, the immunological and toxicological changes by the difference in MOG(35-55) properties modulate EAE induction, and MOG(35-55) which affects MMP-9 activity and IL-6 levels may be the most effective EAE-inducing antigen. This study can be potentially applied by researchers using MOG(35-55) peptide and manufacturers for MOG(35-55) synthesis. English SPRINGER/PLENUM PUBLISHERS Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG(35-55) Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6 Article 10.1007/s11064-015-1732-9 1 NEUROCHEMICAL RESEARCH, v.41, no.4, pp.666 - 676 NEUROCHEMICAL RESEARCH 41 4 666 676 N scie scopus 000373860300005 2-s2.0-84944691148 Biochemistry & Molecular Biology Neurosciences Biochemistry & Molecular Biology Neurosciences & Neurology Article BLOOD-BRAIN-BARRIER MYELIN OLIGODENDROCYTE GLYCOPROTEIN T-CELL RESPONSES MULTIPLE-SCLEROSIS ANIMAL-MODELS MICE EAE AUTOANTIGEN EXPRESSION MIGRATION MOG(35-55) EAE MMP-9 IL-6 Property Induction