Han, Myoung-Sik Han, Im-Ho Lee, Dahae An, Jun Min Kim, Su-Nam Shin, Myoung-Sook Yamabe, Noriko Hwang, Gwi Seo Yoo, Hye Hyun Choi, Suk-Jung Kang, Ki Sung Jang, Hyuk-Jai 2024-01-20T04:32:58Z 2024-01-20T04:32:58Z 2021-09-04 2016-04 1226-8453 https://pubs.kist.re.kr/handle/201004/124245 Background: Nephrotoxicity is a common side effect of medications. Panax ginseng is one of the best-known herbal medicines, and its individual constituents enhance renal function. Identification of its efficacy and mechanisms of action against drug-induced nephrotoxicity, as well as the specific constituents mediating this effect, have recently emerged as an interesting research area focusing on the kidney protective efficacy of P. ginseng. Methods: The present study investigated the kidney protective effect of fermented black ginseng (FBG) and its active component ginsenoside 20(S)-Rg3 against cisplatin (chemotherapy drug)-induced damage in pig kidney (LLC-PK1) cells. It focused on assessing the role of mitogen-activated protein kinases as important mechanistic elements in kidney protection. Results: The reduced cell viability induced by cisplatin was significantly recovered with FBG extract and ginsenoside 20(S)-Rg3 dose-dependently. The cisplatin-induced elevated protein levels of phosphorylated c-Jun N-terminal kinase (JNK), p53, and cleaved caspase-3 were decreased after cotreatment with FBG extract or ginsenoside 20(S)-Rg3. The elevated percentage of apoptotic LLC-PK1 cells induced by cisplatin treatment was significantly abrogated by cotreatment with FBG and the ginsenoside 20(S)-Rg3. Conclusion: FBG and its major ginsenoside 20(S)-Rg3, ameliorated cisplatin-induced nephrotoxicity in LLC-PK1 cells by blocking the JNK-p53-caspase-3 signaling cascade. Copyright 2015, The Korean Society of Ginseng, Published by Elsevier. English KOREAN SOC GINSENG NECROSIS-FACTOR-ALPHA OXIDATIVE STRESS DNA-DAMAGE PANAX-GINSENG RENAL DAMAGE IN-VITRO GENTAMICIN INHIBITION APOPTOSIS TOXICITY Beneficial effects of fermented black ginseng and its ginsenoside 20(S)-Rg3 against cisplatin-induced nephrotoxicity in LLC-PK1 cells Article 10.1016/j.jgr.2015.06.006 1 JOURNAL OF GINSENG RESEARCH, v.40, no.2, pp.135 - 140 JOURNAL OF GINSENG RESEARCH 40 2 135 140 scie scopus kci ART002100147 000373607900006 2-s2.0-85015546858 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article NECROSIS-FACTOR-ALPHA OXIDATIVE STRESS DNA-DAMAGE PANAX-GINSENG RENAL DAMAGE IN-VITRO GENTAMICIN INHIBITION APOPTOSIS TOXICITY cisplatin ginsenoside 20(S)-Rg3 mitogen-activated protein kinases nephrotoxicity Panax ginseng