Thai, Hien Bao Dieu Yu, Jin Kyung Park, Byung Sun Park, Yeon-Joon Min, Sun-Joon Ahn, Dae-Ro 2024-01-20T04:33:45Z 2024-01-20T04:33:45Z 2022-01-10 2016-03-15 0956-5663 https://pubs.kist.re.kr/handle/201004/124283 We devised and synthesized a fluorogenic substrate of beta-lactamases as a probe to detect the activity of the enzymes. Fluorescence of the probe emitted upon treatment of a beta-lactamase and increased proportionally to the concentration of the enzyme, demonstrating its sensing property for the activity of the enzyme. We also showed that the probe could be utilized to assay the enzyme and to determine kinetic parameters of the enzyme. Moreover, the probe was able to detect resistance to the third-generation oxyimino-cephalosporin-derived antibiotics such as cefotaxime and ceftazidime. In particular, the probe could identify the ceftazidime-resistance in bacteria that was not detectable using conventional pH-sensing materials, indicating the practical utility of the probe. (C) 2015 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY GENE-EXPRESSION RAPID DETECTION REPORTER SENSOR A fluorogenic substrate of beta-lactamases and its potential as a probe to detect the bacteria resistant to the third-generation oxyimino-cephalosporins Article 10.1016/j.bios.2015.10.081 1 BIOSENSORS & BIOELECTRONICS, v.77, pp.1026 - 1031 BIOSENSORS & BIOELECTRONICS 77 1026 1031 scie scopus 000366766900145 2-s2.0-84946423711 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article GENE-EXPRESSION RAPID DETECTION REPORTER SENSOR beta-lactamases Fluorogenic probe Antibiotic resistance Fluorescence intensity Oxyimino-cephalosporins