Jung, Seungwon Kim, Junsun Lee, Dong Jin Oh, Eun Hae Lim, Hwasup Kim, Kwang Pyo Choi, Nakwon Kim, Tae Song Kim, Sang Kyung 2024-01-20T04:33:49Z 2024-01-20T04:33:49Z 2021-09-04 2016-03-11 2045-2322 https://pubs.kist.re.kr/handle/201004/124287 Multiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has received significant attention as a mean of verifying the rapidly increasing genetic targets of interest in single phenotype. Here we suggest a readily extensible qPCR for the expression analysis of multiple microRNA (miRNA) targets using microparticles of primer-immobilized networks as discrete reactors. Individual particles, 200 similar to 500 mu m in diameter, are identified by two-dimensional codes engraved into the particles and the non-fluorescent encoding allows high-fidelity acquisition of signal in real-time PCR. During the course of PCR, the amplicons accumulate in the volume of the particles with high reliability and amplification efficiency over 95%. In a quick assay comprising of tens of particles holding different primers, each particle brings the independent real-time amplification curve representing the quantitative information of each target. Limited amount of sample was analyzed simultaneously in single chamber through this highly multiplexed qPCR; 10 kinds of miRNAs from purified extracellular vesicles (EVs). English NATURE PUBLISHING GROUP QUANTIFICATION PARTICLES Extensible Multiplex Real-time PCR of MicroRNA Using Microparticles Article 10.1038/srep22975 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000371776500002 2-s2.0-84960928305 Multidisciplinary Sciences Science & Technology - Other Topics Article QUANTIFICATION PARTICLES 분자진단 다중마커 진단 실시간 PCR 입자기반 qPCR miRNA 패턴인식 입자식별