Kim, Sooji Jeon, Jae-Ok Jun, Eunsung Jee, JunGoo Jung, Hyun-Kyung Lee, Byung-Heon Kim, In-San Kim, Soyoun 2024-01-20T05:00:24Z 2024-01-20T05:00:24Z 2021-09-04 2016-03 1525-7797 https://pubs.kist.re.kr/handle/201004/124349 Ferritin cage nanoparticles are promising platforms for targeted delivery of imaging and therapeutic agents because their cage structure can accommodate small molecules and their surfaces can be decorated with multiple functionalities. However, selective targeting is still a challenge for translating ferritin-based nanomedicines into the clinic, especially for heterogeneous diseases such as cancer. Targeting peptides can be genetically fused onto the surface of a ferritin cage, forming peptide bunches on nanocages (PBNCs) that offer synergistic increases in binding avidity. Here, we utilized two sites of the ferritin monomer, the N-terminus and the loop between the fourth and fifth helices, which are exposed on the surface of the assembled 24-subunit ferritin cage, to ligate one or two types of peptides to achieve super affinity and bispecificity, respectively. PBNCs formed by ligation of the IL-4 receptor-targeting peptide, AP1, to both sites (48AP1-PBNCs) tethered IL-4R, expressing tumor cells with greater affinity than did PBNCs with AP1 ligated to a single site (24AP1-PBNCs). Moreover, bispecific PBNCs containing 24 RGD peptides and 24 AP1 peptides (24RGD/24AP1-PBNCs) were capable of independently targeting cells expressing the corresponding receptors. Bispecific and superaffinity PBNCs could be useful for efficient targeting of ferritin-based therapeutic/diagnostic agents in a clinical setting. English AMER CHEMICAL SOC METAL-ION BINDING DRUG-DELIVERY NANOPARTICLE VACCINES CAGE NANOPARTICLES ENHANCED DELIVERY PROTEIN CAGE FERRITIN CANCER TUMOR PLATFORM Designing Peptide Bunches on Nanocage for Bispecific or Superaffinity Targeting Article 10.1021/acs.biomac.5b01753 1 BIOMACROMOLECULES, v.17, no.3, pp.1150 - 1159 BIOMACROMOLECULES 17 3 1150 1159 scie scopus 000372391800048 2-s2.0-84960903811 Biochemistry & Molecular Biology Chemistry, Organic Polymer Science Biochemistry & Molecular Biology Chemistry Polymer Science Article METAL-ION BINDING DRUG-DELIVERY NANOPARTICLE VACCINES CAGE NANOPARTICLES ENHANCED DELIVERY PROTEIN CAGE FERRITIN CANCER TUMOR PLATFORM