Park, Miri Song, Chiman Yoon, Hojong Choi, Kee-Hyun 2024-01-20T05:00:26Z 2024-01-20T05:00:26Z 2021-09-04 2016-03 1948-7193 https://pubs.kist.re.kr/handle/201004/124351 Glioblastoma is the most common and aggressive primary malignant brain tumor. Temozolomide (TMZ), a chemotherapeutic agent combined with radiation therapy, is used as a standard treatment. The infiltrative nature of glioblastoma, however, interrupts effective treatment with TMZ and increases the tendency to relapse. Voltage-gated chloride channels have been identified as crucial regulators of glioma cell migration and invasion by mediating cell shape and volume change. Accordingly, chloride current inhibition by 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), a chloride channel blocker, suppresses cell movement by diminishing the osmotic cell volume regulation. In this study, we developed a novel compound, TMZ conjugated with NPPB (TMZ-NPPB), as a potential anticancer drug. TMZ-NPPB blocked chloride currents in U373MG, a severely invasive human glioma cell line, and suppressed migration and invasion of U373MG cells. Moreover, TMZ-NPPB exhibited DNA modification activity similar to that of TMZ, and surprisingly showed remarkably enhanced cytotoxicity relative to TMZ by inducing apoptotic cell death via DNA damage. These findings indicate that TMZ-NPPB has a dual function in blocking both proliferation and migration of human glioma cells, thereby suggesting its potential to overcome challenges in current glioblastoma therapy. English AMER CHEMICAL SOC Double Blockade of Glioma Cell Proliferation and Migration by Temozolomide Conjugated with NPPB, a Chloride Channel Blocker Article 10.1021/acschemneuro.5b00178 1 ACS CHEMICAL NEUROSCIENCE, v.7, no.3, pp.275 - 285 ACS CHEMICAL NEUROSCIENCE 7 3 275 285 N scie scopus 000372479600003 2-s2.0-84962292009 Biochemistry & Molecular Biology Chemistry, Medicinal Neurosciences Biochemistry & Molecular Biology Pharmacology & Pharmacy Neurosciences & Neurology Article COMET ASSAY DNA-DAMAGE ANTITUMOR IMIDAZOTETRAZINES PERILLYL ALCOHOL INVASION AGENTS GROWTH CONDUCTANCE ACTIVATION CURRENTS Temozolomide chloride channel blocker NPPB glioblastoma voltage-gated chloride channel migration proliferation