Jung, Jae Hee Lee, Jung Eun 2024-01-20T05:00:54Z 2024-01-20T05:00:54Z 2021-09-05 2016-02-23 2045-2322 https://pubs.kist.re.kr/handle/201004/124373 Observing microbial colonies is the standard method for determining the microbe titer and investigating the behaviors of microbes. Here, we report an automated, real-time bacterial microcolony-counting system implemented on a wide field-of-view (FOV), on-chip microscopy platform, termed ePetri. Using sub-pixel sweeping microscopy (SPSM) with a super-resolution algorithm, this system offers the ability to dynamically track individual bacterial microcolonies over a wide FOV of 5.7 mm x 4.3 mm without requiring a moving stage or lens. As a demonstration, we obtained high-resolution time-series images of S. epidermidis at 20-min intervals. We implemented an imageprocessing algorithm to analyze the spatiotemporal distribution of microcolonies, the development of which could be observed from a single bacterial cell. Test bacterial colonies with a minimum diameter of 20 mu m could be enumerated within 6 h. We showed that our approach not only provides results that are comparable to conventional colony-counting assays but also can be used to monitor the dynamics of colony formation and growth. This microcolony-counting system using on-chip microscopy represents a new platform that substantially reduces the detection time for bacterial colony counting. It uses chipscale image acquisition and is a simple and compact solution for the automation of colony-counting assays and microbe behavior analysis with applications in antibacterial drug discovery. English NATURE PUBLISHING GROUP FIELD-OF-VIEW COLONIES RECONSTRUCTION SYSTEM Real-time bacterial microcolony counting using on-chip microscopy Article 10.1038/srep21473 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000370626000001 2-s2.0-84959378052 Multidisciplinary Sciences Science & Technology - Other Topics Article FIELD-OF-VIEW COLONIES RECONSTRUCTION SYSTEM Bioaerosol Microcolony Colony Counting OnChip