Park, Dong Jin Min, Kyung Hyun Lee, Hong Jae Kim, Kwangmeyung Kwon, Ick Chan Jeong, Seo Young Lee, Sang Cheon 2024-01-20T05:01:49Z 2024-01-20T05:01:49Z 2022-01-25 2016-02 2050-7518 https://pubs.kist.re.kr/handle/201004/124420 In this work, we have developed photosensitizer-loaded bubble-generating calcium carbonate (CaCO3)-mineralized nanoparticles that have potential for ultrasound imaging (US)-guided photodynamic therapy (PDT) of tumors. A photosensitizer, chlorin e6 (Ce6)-loaded CaCO3-mineralized nanoparticles (Ce6-BMNs), was prepared using an anionic block copolymer-templated in situ mineralization method. Ce6-BMNs were composed of the Ce6-loaded CaCO3 core and the hydrated poly(ethylene glycol) (PEG) shell. Ce6-BMNs exhibited excellent stability under serum conditions. Ce6-BMNs showed enhanced echogenic US signals at tumoral acid pH by generating carbon dioxide (CO2) bubbles. Ce6-BMNs effectively inhibited Ce6 release at physiological pH (7.4). At a tumoral acidic pH (6.4), Ce6 release was accelerated with CO2 bubble generation due to the dissolution of the CaCO3 mineral core. Upon irradiation of Ce6-BMN-treated MCF-7 breast cancer cells, the cell viability dramatically decreased with increasing Ce6 concentration. The phototoxicity of the Ce6-BMNs was much higher than that of free Ce6. On the basis of tumoral pH-responsive CO2 bubble-generation and simultaneous Ce6 release at the target tumor site, these CaCO3 mineralized nanoparticles can be considered as promising theranostic nanoparticles for US imaging-guided PDT in the field of tumor therapy. English ROYAL SOC CHEMISTRY Photosensitizer-loaded bubble-generating mineralized nanoparticles for ultrasound imaging and photodynamic therapy Article 10.1039/c5tb02338f 1 JOURNAL OF MATERIALS CHEMISTRY B, v.4, no.7, pp.1219 - 1227 JOURNAL OF MATERIALS CHEMISTRY B 4 7 1219 1227 N scie scopus 000370427400003 2-s2.0-84957998604 Materials Science, Biomaterials Materials Science Article MICROBUBBLE CONTRAST AGENTS DRUG-DELIVERY IN-VITRO TUMOR ANGIOGENESIS CONTROLLED-RELEASE MESOPOROUS SILICA THERANOSTIC AGENT CANCER-TREATMENT HYALURONIC-ACID BLOCK-COPOLYMER