Choi, Bogyu Park, Kwang-Sook Kim, Ji-Ho Ko, Kyoung-Won Kim, Jin-Su Han, Dong Keun Lee, Soo-Hong 2024-01-20T05:02:23Z 2024-01-20T05:02:23Z 2021-09-05 2016-02 1616-5187 https://pubs.kist.re.kr/handle/201004/124449 The stiffness of hydrogels has been reported to direct cell fate. Here, we found that the stiffness of hydrogels promotes the reprogramming of mouse embryonic fibroblasts into induced pluripotent stem cells (iPSCs). We prepared cell culture substrates of various stiffnesses (0.1, 1, 4, 10, and 20kPa) using a polyacrylamide hydrogel. We found that culture on a soft hydrogel plays an important role in inducing cellular reprogramming into iPSCs via activation of mesenchymal-to-epithelial transition and enhancement of stemness marker expression. These results suggest that physical signals at the interface between cell and substrate can be used as a potent regulator to promote cell fate changes associated with reprogramming into iPSCs, which may lead to effective and reproducible iPSC-production. English WILEY-V C H VERLAG GMBH E-CADHERIN SUBSTRATE STIFFNESS MATRIX STIFFNESS N-CADHERIN CELLS MOUSE EMT APOPTOSIS SWITCH OCT4 Stiffness of Hydrogels Regulates Cellular Reprogramming Efficiency Through Mesenchymal-to-Epithelial Transition and Stemness Markers Article 10.1002/mabi.201500273 1 MACROMOLECULAR BIOSCIENCE, v.16, no.2, pp.199 - 206 MACROMOLECULAR BIOSCIENCE 16 2 199 206 scie scopus 000370083000004 2-s2.0-84958046653 Biochemistry & Molecular Biology Materials Science, Biomaterials Polymer Science Biochemistry & Molecular Biology Materials Science Polymer Science Article E-CADHERIN SUBSTRATE STIFFNESS MATRIX STIFFNESS N-CADHERIN CELLS MOUSE EMT APOPTOSIS SWITCH OCT4 cellular reprogramming induced pluripotent stem cells mesenchymal-to-epithelial transition stemness markers stiffness of hydrogel