Lee, Eunsoo Choi, Jungyoon Jo, Youhwa Kim, Joo Yeon Jang, Yu Jin Lee, Hye Myeong Kim, So Yeun Lee, Ho-Jae Cho, Keunchang Jung, Neoncheol Hur, Eun Mi Jeong, Sung Jin Moon, Cheil Choe, Youngshik Rhyu, Im Joo Kim, Hyun Sun, Woong 2024-01-20T05:03:29Z 2024-01-20T05:03:29Z 2021-09-05 2016-01-11 2045-2322 https://pubs.kist.re.kr/handle/201004/124508 Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies. English NATURE PUBLISHING GROUP SINGLE-CELL RESOLUTION MOUSE-BRAIN TOMOGRAPHY AGENT ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging Article 10.1038/srep18631 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000368127400001 2-s2.0-84954117609 Multidisciplinary Sciences Science & Technology - Other Topics Article SINGLE-CELL RESOLUTION MOUSE-BRAIN TOMOGRAPHY AGENT