Kim, Hea-Jin Koh, Hae-Young 2024-01-20T05:03:34Z 2024-01-20T05:03:34Z 2021-09-05 2016-01-05 1932-6203 https://pubs.kist.re.kr/handle/201004/124513 Hallucinations and delusions are the most prominent symptoms of schizophrenia and characterized by impaired reality testing. Representation-mediated taste aversion (RMTA) has been proposed as a potential behavioral assessment of reality testing and has been applied to a neurodevelopmental rat model of schizophrenia. However, the theory underlying this approach has not been generalized yet with any demonstration of impaired reality testing in other animal models of schizophrenia, such as genetically-modified mice. We devised a RMTA procedure for mice that combines a Pavlovian association protocol pairing odor conditioned stimulus (CS) with sugar reward unconditioned stimulus (US), and a conditioned taste aversion (CTA) method. In this RMTA paradigm, we compared performances of wildtype (PLC beta 1(+/+)) mice and phospholipase C beta 1 knock-out (PLC beta 1(-/-)) mice which are known as one of the genetic models for schizophrenia. With a minimal amount of initial odor-sugar associative training, both PLC beta 1(+/+) and PLC beta 1(-/-) mice were able to form an aversion to the sugar reward when the odor CS predicting sugar was paired with nausea. With an extended initial training, however, only PLC beta 1(-/-) mice could form a RMTA. This persistent RMTA displayed by PLC beta 1(-/-) mice shows their inability to distinguish real sugar from the CS-evoked representation of sugar at a stage in associative learning where wild-type mice normally could differentiate the two. These results demonstrate an impaired reality testing first observed in a genetic mouse model of schizophrenia, and suggest that RMTA paradigm may, with general applicability, allow diverse biological approaches to impaired reality testing. English PUBLIC LIBRARY SCIENCE AUDITORY VERBAL HALLUCINATIONS EVENT REPRESENTATION KNOCKOUT MICE SCHIZOPHRENIA MODEL ENDOPHENOTYPES STIMULUS DISEASE MEMORY BRAIN Impaired Reality Testing in Mice Lacking Phospholipase C beta 1: Observed by Persistent Representation-Mediated Taste Aversion Article 10.1371/journal.pone.0146376 1 PLOS ONE, v.11, no.1 PLOS ONE 11 1 scie scopus 000367801400164 2-s2.0-84954122095 Multidisciplinary Sciences Science & Technology - Other Topics Article AUDITORY VERBAL HALLUCINATIONS EVENT REPRESENTATION KNOCKOUT MICE SCHIZOPHRENIA MODEL ENDOPHENOTYPES STIMULUS DISEASE MEMORY BRAIN hallucination impaired reality testing mice phospholipase Cβ1 representation mediated learning schizophrenia