Pai, Jaeyoung Hyun, Soonsil Hyun, Ji Young Park, Seong-Hyun Kim, Won-Je Bae, Sung-Hun Kim, Nak-Kyoon Yu, Jaehoon Shin, Injae 2024-01-20T05:04:01Z 2024-01-20T05:04:01Z 2021-09-05 2016-01 0002-7863 https://pubs.kist.re.kr/handle/201004/124538 MicroRNA-155, one of the most potent miRNAs that suppress apoptosis in human cancer, is overexpressed in numerous cancers, and it displays oncogenic activity. Peptide microarrays, constructed by immobilizing 185 peptides containing the C-terminal hydrazide onto epoxide-derivatized glass slides, were employed to evaluate peptide binding properties of pre-miRNA-155 and to identify its binding peptides. Two peptides, which were identified based on the results of peptide microarray and in vitro Dicer inhibition studies, were found to inhibit generation of mature miRNA-155 catalyzed by Dicer and to enhance expression of miRNA-155 target genes in cells. In addition, the results of cell experiments indicate that peptide inhibitors promote apoptotic cell death via a caspase-dependent pathway. Finally, observations made in NMR and molecular modeling studies suggest that a peptide inhibitor preferentially binds to the upper bulge and apical stem-loop region of pre-miRNA-155, thereby suppressing Dicer-mediated miRNA-155 processing. English AMER CHEMICAL SOC Screening of Pre-miRNA-155 Binding Peptides for Apoptosis inducing Activity Using Peptide Microarrays Article 10.1021/jacs.5b09216 1 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.138, no.3, pp.857 - 867 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138 3 857 867 N scie scopus 000369044400030 2-s2.0-84961335756 Chemistry, Multidisciplinary Chemistry Article SMALL-MOLECULE BREAST-CANCER RNA STRUCTURE POSTTRANSCRIPTIONAL REGULATION HELICAL PEPTIDES IN-VIVO MICRORNA EXPRESSION SEQUENCE COMPLEX HSP70 Apoptozole Pre-miRNA-155 Peptide microarray NMR Apoptosis miRNA RNA structure model