Bang, Seokyoung Na, Sangcheol Jang, Jae Myung Kim, Jinhyun Jeon, Noo Li 2024-01-20T05:04:17Z 2024-01-20T05:04:17Z 2021-09-03 2016-01 2192-2640 https://pubs.kist.re.kr/handle/201004/124553 The brain is one of the most important and complex organs in the human body. Although various neural network models have been proposed for in vitro 3D neuronal networks, it has been difficult to mimic functional and structural complexity of the in vitro neural circuit. Here, a microfluidic model of a simplified 3D neural circuit is reported. First, the microfluidic device is filled with Matrigel and continuous flow is delivered across the device during gelation. The fluidic flow aligns the extracellular matrix (ECM) components along the flow direction. Following the alignment of ECM fibers, neurites of primary rat cortical neurons are grown into the Matrigel at the average speed of 250 mu m d(-1) and form axon bundles approximately 1500 mu m in length at 6 days in vitro (DIV). Additionally, neural networks are developed from presynaptic to postsynaptic neurons at 14 DIV. The establishment of aligned 3D neural circuits is confirmed with the immunostaining of PSD-95 and synaptophysin and the observation of calcium signal transmission. English WILEY CELL-MIGRATION IN-VITRO CULTURES GROWTH ORGANS Engineering-Aligned 3D Neural Circuit in Microfluidic Device Article 10.1002/adhm.201500397 1 ADVANCED HEALTHCARE MATERIALS, v.5, no.1, pp.159 - 166 ADVANCED HEALTHCARE MATERIALS 5 1 159 166 scie scopus 000368144200014 2-s2.0-84953839494 Engineering, Biomedical Nanoscience & Nanotechnology Materials Science, Biomaterials Engineering Science & Technology - Other Topics Materials Science Article CELL-MIGRATION IN-VITRO CULTURES GROWTH ORGANS 3D neuron cultures Axon fasciculation In vitro neural circuit Microfluidics