김원제 김낙균 2024-01-20T05:31:52Z 2024-01-20T05:31:52Z 2021-09-06 2015-12 1226-6531 https://pubs.kist.re.kr/handle/201004/124668 In some pathogenic bacteria, there are RNA thermometers, which regulate the production of virulence associated factors or heat shock proteins depending on temperature changes. Like a riboswitches, RNA thermometers are located in the 5’-untranslated region and involved translational gene regulatory mechanism. RNA thermometers block the ribosome-binding site and start codon area under the 37℃ within their secondary structure. After bacterial infection, increased the temperature in the host causes conformations changes of RNA, and the ribosome-binding site is exposed for translational initiation. Because structural differences between open and closed forms of RNA thermometers are mainly mediated by base pairing changes, NMR spectroscopy is a very useful method to study these thermodynamically changing RNA structure. In this review, we briefly provide a fundamental function of RNA thermometers, and also suggest a proper NMR experiments for studying RNA thermometers. English 한국자기공명학회 Proper NMR methods for studying RNA thermometers Article 10.6564/JKMRS.2015.19.3.143 2 Journal of the Korean Magnetic Resonance Society, v.19, no.3, pp.143 - 148 Journal of the Korean Magnetic Resonance Society 19 3 143 148 kci ART002056791 RNA thermometer NMR methods thermodynamic structure