Cleland, Timothy P. Schroeter, Elena R. Zamdborg, Leonid Zheng, Wenxia Lee, Ji Eun Tran, John C. Bern, Marshall Duncan, Michael B. Lebleu, Valerie S. Ahlf, Dorothy R. Thomas, Paul M. Kalluri, Raghu Kelleher, Neil L. Schweitzer, Mary H. 2024-01-20T05:32:27Z 2024-01-20T05:32:27Z 2021-09-03 2015-12 1535-3893 https://pubs.kist.re.kr/handle/201004/124700 Structures similar to blood vessels in location, morphology, flexibility, and transparency have been recovered after demineralization of multiple dinosaur cortical bone fragments from multiple specimens, some of which are as old as 80 Ma. These structures were hypothesized to be either endogenous to the bone (i.e., of vascular origin) or the result of biofilm colonizing the empty osteonal network after degradation of original organic components. Here, we test the hypothesis that these structures are endogenous and thus retain proteins in common with extant archosaur blood vessels that can be detected with high-resolution mass spectrometry and confirmed by irnmunofluorescence. Two lines of evidence support this hypothesis. First, peptide sequencing of Brachylophosaurus canadensis blood vessel extracts is consistent with peptides comprising extant archosaurian blood vessels and is not consistent with a bacterial, cellular slime mold, or fungal origin. Second, proteins identified by mass spectrometry can be localized to the tissues using antibodies specific to these proteins, validating their identity. Data are available via ProteomeXchange with identifier PXDO01738. English AMER CHEMICAL SOC PROTEIN SEQUENCES ARCHAEOLOGICAL BONE OSTEOCALCIN PROTEIN FUNGAL HYPHAE ANCIENT BONE SOFT-TISSUE PRESERVATION IDENTIFICATION COLLAGEN OSTEOCYTES Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis Article 10.1021/acs.jproteome.5b00675 1 JOURNAL OF PROTEOME RESEARCH, v.14, no.12, pp.5252 - 5262 JOURNAL OF PROTEOME RESEARCH 14 12 5252 5262 scie scopus 000366151800029 2-s2.0-84948968902 Biochemical Research Methods Biochemistry & Molecular Biology Article PROTEIN SEQUENCES ARCHAEOLOGICAL BONE OSTEOCALCIN PROTEIN FUNGAL HYPHAE ANCIENT BONE SOFT-TISSUE PRESERVATION IDENTIFICATION COLLAGEN OSTEOCYTES Brachylophosaurus canadensis blood vessels dinosaur cytoskeleton actin tubulin myosin tropomyosin tap honomy preservation