Lee, Jin-Ho Kim, Byung-Chan Oh, Byeung-Keun Choi, Jeong-Woo 2024-01-20T05:32:41Z 2024-01-20T05:32:41Z 2021-09-03 2015-12 1550-7033 https://pubs.kist.re.kr/handle/201004/124713 In this study, rapid and sensitive detection of human immunodeficiency virus (HIV)-1 was performed based on immunoreactions with an Au nanodot fabricated indium tin oxide (ITO) substrate using surface-enhanced Raman spectroscopy (SERS). Highly ordered Au nanodots (ca. 20 nm) were electrochemically fabricated over a large surface area (20 mm x 10 mm) of an ITO substrate using a simple deposition method with Triton X-100. On the Au nanodot surface, monoclonal antibody fragments against gp120 were selectively bound by gold-sulfur interactions. Various concentrations (35 fg/mL to 350 pg/mL) of HIV-1 virus-like particles (HIV-1 VLPs) were used for the measurements. The presence of HIV-1 VLPs was rapidly (within 5 s) and successfully determined by SERS due to specific immunoreactions on the Au nanodots without the use of labeling probes. The results showed the possibility of using SERS-related methods as a new immunoassay for the study of biomolecular interactions and detection of low viral loads. Moreover, based on its high sensitivity and chemical specificity, SERS could be used as a promising clinical tool for detecting infectious small biological components. English AMER SCIENTIFIC PUBLISHERS PLASMON RESONANCE VIBRATIONAL ANALYSIS SILVER ELECTRODE PROTEINS RNA NANOPARTICLES IMMUNOSENSOR POLYPEPTIDES ANTIBODIES INFECTION Rapid and Sensitive Determination of HIV-1 Virus Based on Surface Enhanced Raman Spectroscopy Article 10.1166/jbn.2015.2117 1 JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.11, no.12, pp.2223 - 2230 JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 11 12 2223 2230 scie scopus 000361931700011 2-s2.0-84928656785 Nanoscience & Nanotechnology Materials Science, Biomaterials Science & Technology - Other Topics Materials Science Article PLASMON RESONANCE VIBRATIONAL ANALYSIS SILVER ELECTRODE PROTEINS RNA NANOPARTICLES IMMUNOSENSOR POLYPEPTIDES ANTIBODIES INFECTION Raman Scattering Virus Au Nanodot HIV-1 Surface-Enhanced Raman Spectroscopy