Yoo, Gu Saenger, Thorsten Bong, Ji-Hong Jose, Joachim Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T05:32:42Z 2024-01-20T05:32:42Z 2021-09-03 2015-12 0005-2736 https://pubs.kist.re.kr/handle/201004/124714 In this work, two proteins, Z-domains and bovine casein, were autodisplayed on the outer membrane of the same Escherichia coli cells by co-transformation of two different autodisplay vectors. On the basis of SDS-PAGE densitometry, Z-domains and bovine casein were expressed at 3.12 x 10(5) and 1.55 x 10(5) proteins/E. coli cell, respectively. The co-autodisplayed Z-domains had antibody-binding activity and the bovine casein had adhesive properties. E. coli with co-autodisplayed proteins were analyzed by fluorescence assisted cell sorting (FACS). E. coli with co-autodisplayed Z-domains and bovine casein aggregated due to hydrophobic interaction. For application to immunoassays, the Z-domain activity was estimated after (1) immobilizing the E. coli and (2) forming an OM layer. E. coli with co-autodisplayed two proteins that were immobilized on a polystyrene microplate had the same antibody-binding activity as did E. coli with autodisplayed Z-domains only. The OM layer from the cotransformed E. coli had Z-domains and bovine casein expressed at a 1:2 ratio from antibody-binding activity measurements. (C) 2015 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV ESCHERICHIA-COLI SURFACE DISPLAY RECOMBINANT PROTEINS SPR BIOSENSOR IMMUNOASSAY ELISA Co-autodisplay of Z-domains and bovine caseins on the outer membrane of E. coli Article 10.1016/j.bbamem.2015.09.018 1 BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v.1848, no.12, pp.3126 - 3133 BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1848 12 3126 3133 scie scopus 000364891300002 2-s2.0-84943763052 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article ESCHERICHIA-COLI SURFACE DISPLAY RECOMBINANT PROTEINS SPR BIOSENSOR IMMUNOASSAY ELISA Autodisplay Z-domain Casein FACS Immunoassay