Yang, Min Jung Ku, Sook Hee Kim, Dongkyu Kim, Won Jong Mok, Hyejung Kim, Sun Hwa Kwon, Ick Chan 2024-01-20T05:32:55Z 2024-01-20T05:32:55Z 2021-09-03 2015-12 1616-5187 https://pubs.kist.re.kr/handle/201004/124726 This study aims to develop bioreducible polyethylenimine (rPEI)/siRNA polyplexes with high stability, high transfection efficiency, and low cytotoxicity for efficient cytoplasmic siRNA delivery. rPEI successfully incorporated siRNA into stable and compact nano-complexes, and the disulfide linkages in rPEI/siRNA were cleaved under reductive environments, resulting in efficient intracellular translocation and siRNA release. In this study, receptor for advanced glycation end-products (RAGE) was selected as a therapeutic target gene because it is associated with inflammatory responses in ischemia/reperfusion injury. rPEI/siRAGE exhibited high target gene silencing and low cytotoxicity in cardiomyocytes, and the treatment of rPEI/siRAGE reduced the myocardial infarction size. English WILEY-V C H VERLAG GMBH TRANSFECTION INJURY Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy Article 10.1002/mabi.201500213 1 MACROMOLECULAR BIOSCIENCE, v.15, no.12, pp.1755 - 1763 MACROMOLECULAR BIOSCIENCE 15 12 1755 1763 scie scopus 000368456500014 2-s2.0-84955177488 Biochemistry & Molecular Biology Materials Science, Biomaterials Polymer Science Biochemistry & Molecular Biology Materials Science Polymer Science Article TRANSFECTION INJURY bioreducible polyethylenimine cytoplasmic delivery myocardial infarction RAGE siRNA