Kyeong, San Jeong, Cheolhwan Kang, Homan Cho, Hong-Jun Park, Sung-Jun Yang, Jin-Kyoung Kim, Sehoon Kim, Hyung-Mo Jun, Bong-Hyun Lee, Yoon-Sik 2024-01-20T05:33:08Z 2024-01-20T05:33:08Z 2021-09-03 2015-11-24 1932-6203 https://pubs.kist.re.kr/handle/201004/124736 Superparamagnetic Fe3O4 nanoparticles (NPs) based nanomaterials have been exploited in various biotechnology fields including biomolecule separation. However, slow accumulation of Fe3O4 NPs by magnets may limit broad applications of Fe3O4 NP-based nanomaterials. In this study, we report fabrication of Fe3O4 NPs double-layered silica nanoparticles (DL MNPs) with a silica core and highly packed Fe3O4 NPs layers. The DL MNPs had a superparamagnetic property and efficient accumulation kinetics under an external magnetic field. Moreover, the magnetic field-exposed DL MNPs show quantitative accumulation, whereas Fe3O4 NPs single-layered silica nanoparticles (SL MNPs) and silica-coated Fe3O4 NPs produced a saturated plateau under full recovery of the NPs. DL MNPs are promising nanomaterials with great potential to separate and analyze biomolecules. English PUBLIC LIBRARY SCIENCE IRON-OXIDE NANOPARTICLES HOLLOW SPHERES DRUG-DELIVERY CELL-SEPARATION QUANTUM DOTS NANOCLUSTERS Double-Layer Magnetic Nanoparticle-Embedded Silica Particles for Efficient Bio-Separation Article 10.1371/journal.pone.0143727 1 PLOS ONE, v.10, no.11 PLOS ONE 10 11 scie scopus 000365862600110 2-s2.0-84957895133 Multidisciplinary Sciences Science & Technology - Other Topics Article IRON-OXIDE NANOPARTICLES HOLLOW SPHERES DRUG-DELIVERY CELL-SEPARATION QUANTUM DOTS NANOCLUSTERS