Kim, Myungsuk Lim, Sue Ji Lee, Hee-Ju Kim, Sun Young Nho, Chu Won 2024-01-20T05:33:31Z 2024-01-20T05:33:31Z 2021-09-03 2015-11-11 0021-8561 https://pubs.kist.re.kr/handle/201004/124755 The aim of our study is to investigate the molecular mechanism of gomisin J from Schisandra chinensis on the oleic acid (OA)-induced lipid accumulation in HepG2 cells. Gomisin J attenuated lipid accumulation in OA-induced HepG2 cells. It also suppressed the expression of lipogenic enzymes and inflammatory mediators and increased the expression of lipolytic enzymes in OA-induced HepG2 cells. Furthermore, the use of specific inhibitors and fetuin-A siRNA and liver kinase B1 (LKB1) siRNA transfected cells demonstrated that gomisin J regulated lipogenesis and lipolysis via inhibition of fetuin-A and activation of an AMP-activated protein kinase (AMPK)-dependent pathway in HepG2 cells. Our results showed that gomisin J suppressed lipid accumulation by regulating the expression of lipogenic and lipolytic enzymes and inflammatory molecules through activation of AMPK, LKB1, and Ca2+/calmodulin-dependent protein kinase II and inhibition of fetuin-A in HepG2 cells. This suggested that gomisin J has potential benefits in treating nonalcoholic fatty liver disease. English AMER CHEMICAL SOC FATTY LIVER-DISEASE SCHISANDRA-CHINENSIS DIBENZOCYCLOOCTADIENE LIGNANS INSULIN-RESISTANCE METABOLIC SYNDROME ADIPOSE-TISSUE PROTEIN-KINASE HYPERCHOLESTEROLEMIC MICE IN-VIVO OBESITY Gomisin J Inhibits Oleic Acid-Induced Hepatic Lipogenesis by Activation of the AMPK-Dependent Pathway and Inhibition of the Hepatokine Fetuin-A in HepG2 Cells Article 10.1021/acs.jafc.5b04089 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.63, no.44, pp.9729 - 9739 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63 44 9729 9739 scie scopus 000364727400008 2-s2.0-84946935085 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article FATTY LIVER-DISEASE SCHISANDRA-CHINENSIS DIBENZOCYCLOOCTADIENE LIGNANS INSULIN-RESISTANCE METABOLIC SYNDROME ADIPOSE-TISSUE PROTEIN-KINASE HYPERCHOLESTEROLEMIC MICE IN-VIVO OBESITY gomisin J AMPK LKB1 CaMKII fetuin-A