Choi, Jeongan Kang, Miran Jung, Jae Hee 2024-01-20T05:33:42Z 2024-01-20T05:33:42Z 2021-09-05 2015-11-02 2045-2322 https://pubs.kist.re.kr/handle/201004/124765 We demonstrate an integrated micro-optofluidic platform for real-time, continuous detection and quantification of airborne microorganisms. Measurements of the fluorescence and light scattering from single particles in a microfluidic channel are used to determine the total particle number concentration and the microorganism number concentration in real-time. The system performance is examined by evaluating standard particle measurements with various sample flow rates and the ratios of fluorescent to non-fluorescent particles. To apply this method to real-time detection of airborne microorganisms, airborne Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis cells were introduced into the micro-optofluidic platform via bioaerosol generation, and a liquid-type particle collection setup was used. We demonstrate successful discrimination of SYTO82-dyed fluorescent bacterial cells from other residue particles in a continuous and real-time manner. In comparison with traditional microscopy cell counting and colony culture methods, this micro-optofluidic platform is not only more accurate in terms of the detection efficiency for airborne microorganisms but it also provides additional information on the total particle number concentration. English NATURE PUBLISHING GROUP ESCHERICHIA-COLI FLOW-CYTOMETRY INDOOR AIR BACTERIA PCR QUANTIFICATION SYSTEM BIOAEROSOLS IMPACTION SYMPTOMS Integrated micro-optofluidic platform for real-time detection of airborne microorganisms Article 10.1038/srep15983 1 SCIENTIFIC REPORTS, v.5 SCIENTIFIC REPORTS 5 scie scopus 000363986100002 2-s2.0-84946762182 Multidisciplinary Sciences Science & Technology - Other Topics Article ESCHERICHIA-COLI FLOW-CYTOMETRY INDOOR AIR BACTERIA PCR QUANTIFICATION SYSTEM BIOAEROSOLS IMPACTION SYMPTOMS Bioaerosols Microfluidics Optofluidics Real-time detection Airborne microorganisms