Gwak, So-Jung Koo, Heebeom Yun, Yeomin Yhee, Ji Young Lee, Hye Yeong Yoon, Do Heum Kim, Kwangmeyung Ha, Yoon 2024-01-20T05:34:26Z 2024-01-20T05:34:26Z 2021-09-05 2015-11 1549-3296 https://pubs.kist.re.kr/handle/201004/124805 Methylprednisolone (MP) is a glucocorticoid that is used as an anti-inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was used to synthesize chitosan-MP conjugate, which was used to evaluate the gene therapy, anti-inflammatory and anti-apoptotic effects of MP. The cytotoxicity of chitosan-MP nanoparticles and the transfection efficiency of plasmid DNA were evaluated by MTT and luciferase assays. A chitosan-MP/pDNA complexes was injected into injured spinal cord to evaluate the anti-inflammatory and anti-apoptotic effects of these complexes using terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) and ED1 staining, respectively. In addition, to evaluate the distribution of chitosan-MP/pDNA complexes, p-gal encapsulated chitosan-MP was injected into the injected site. Cell survival was similar in cells treated with chitosan-MP conjugate and untreated cells. Luciferase expression was higher in cells treated with the chitosan-MP/pDNA than cells treated with the chitosan/pDNA. The chitosan-MP/pDNA complexes also reduced apoptosis and inflammation at the injury site. These results suggest that chitosan-MP conjugation is an effective gene delivery system to treat SCI. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3474-3482, 2015. English WILEY NUCLEAR-LOCALIZATION SIGNAL IN-VIVO FUNCTIONAL RECOVERY METHYLPREDNISOLONE CHITOSAN TRANSFECTION THERAPY CELLS CARRIERS VECTORS Multifunctional nanoparticles for gene delivery and spinal cord injury Article 10.1002/jbm.a.35489 1 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v.103, no.11, pp.3474 - 3482 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103 11 3474 3482 scie scopus 000362221700006 2-s2.0-84942836354 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article NUCLEAR-LOCALIZATION SIGNAL IN-VIVO FUNCTIONAL RECOVERY METHYLPREDNISOLONE CHITOSAN TRANSFECTION THERAPY CELLS CARRIERS VECTORS methylprednisolone chitosan spinal cord injury gene delivery