Shin, Jonghyeok Jung, Young-Hun Cho, Da-Hyeong Park, Myungseo Lee, Kyung Eun Yang, Yoosoo Jeong, Cherlhyun Sung, Bong Hyun Sohn, Jung-Hoon Park, Jin-Byung Kweon, Dae-Hyuk 2024-01-20T06:00:32Z 2024-01-20T06:00:32Z 2021-09-04 2015-11 0141-0229 https://pubs.kist.re.kr/handle/201004/124845 Caveolae are membrane-budding structures that exist in many vertebrate cells. One of the important functions of caveolae is to form membrane curvature and endocytic vesicles. Recently, it was shown that caveolae-like structures were formed in Escherichia coli through the expression of caveolin-1. This interesting structure seems to be versatile for a variety of biotechnological applications. Targeting of heterologous proteins in the caveolae-like structure should be the first question to be addressed for this purpose. Here we show that membrane proteins co-expressed with caveolin-1 are embedded into the heterologous caveolae (h-caveolae), the cavaolae-like structures formed inside the cell. Two transmembrane SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, Syntaxin la and vesicle-associated membrane protein 2 (VAMP2), were displayed on the h-caveolae surface. The size of the h-caveolae harboring the transmembrane proteins was similar to 100 nm in diameter. The proteins were functional and faced outward on the h-caveolae. Multi-spanning transmembrane proteins FtsH and FeoB could be included in the h-caveolae, too. Furthermore, the recombinant E. coli cells were shown to endocytose substrate supplemented in the medium. These results provide a basis for exploiting the h-caveolae formed inside E. coli cells for future biotechnological applications. (C) 2015 Elsevier Inc. All rights reserved. English ELSEVIER SCIENCE INC PHOSPHOLIPID-VESICLES EXPRESSION RECONSTITUTION FUSION PURIFICATION AGGREGATION BACTERIUM COMPLEX Display of membrane proteins on the heterologous caveolae carved by caveolin-1 in the Escherichia coli cytoplasm Article 10.1016/j.enzmictec.2015.06.018 1 ENZYME AND MICROBIAL TECHNOLOGY, v.79-80, pp.55 - 62 ENZYME AND MICROBIAL TECHNOLOGY 79-80 55 62 scie scopus 000361935100008 2-s2.0-84938085298 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article PHOSPHOLIPID-VESICLES EXPRESSION RECONSTITUTION FUSION PURIFICATION AGGREGATION BACTERIUM COMPLEX Caveolin-1 Heterologous caveolae Transmembrane protein