Han, Hyung-Seop Kim, Hee-Kyoung Kim, Yu-Chan Seok, Hyun-Kwang Kim, Young-Yul 2024-01-20T06:00:33Z 2024-01-20T06:00:33Z 2021-09-04 2015-11 1598-9623 https://pubs.kist.re.kr/handle/201004/124846 Unique biodegradable property of magnesium has spawned countless studies to develop ideal biodegradable orthopedic implant materials in the last decade. However, due to the rapid pH change and extensive amount of hydrogen gas generated during biocorrosion, it is extremely difficult to determine the accurate cytotoxicity of newly developed magnesium alloys using the existing methods. Herein, we report a new method to accurately determine the cytotoxicity of magnesium alloys with varying corrosion rate while taking in-vivo condition into the consideration. For conventional method, extract quantities of each metal ion were determined using ICP-MS and the result showed that the cytotoxicity due to pH change caused by corrosion affected the cell viability rather than the intrinsic cytotoxicity of magnesium alloy. In physiological environment, pH is regulated and adjusted within normal pH (similar to 7.4) range by homeostasis. Two new methods using pH buffered extracts were proposed and performed to show that environmental buffering effect of pH, dilution of the extract, and the regulation of eluate surface area must be taken into consideration for accurate cytotoxicity measurement of biodegradable magnesium alloys. English KOREAN INST METALS MATERIALS IN-VITRO DEGRADATION MECHANICAL-PROPERTIES CORROSION IMPLANT RELEASE MICROSTRUCTURE SCAFFOLDS BEHAVIOR GROWTH PH Conventional and improved cytotoxicity test methods of newly developed biodegradable magnesium alloys Article 10.1007/s12540-015-5069-3 1 METALS AND MATERIALS INTERNATIONAL, v.21, no.6, pp.1108 - 1117 METALS AND MATERIALS INTERNATIONAL 21 6 1108 1117 scie scopus kci ART002045137 000363851100018 2-s2.0-84945282080 Materials Science, Multidisciplinary Metallurgy & Metallurgical Engineering Materials Science Metallurgy & Metallurgical Engineering Article IN-VITRO DEGRADATION MECHANICAL-PROPERTIES CORROSION IMPLANT RELEASE MICROSTRUCTURE SCAFFOLDS BEHAVIOR GROWTH PH biomaterials biocompatibility corrosion cytotoxicity metals