Lee, Jee-Wook Ok, Myoung-Ryul Lee, Sangmook Lim, Jin Ik 2024-01-20T06:00:41Z 2024-01-20T06:00:41Z 2021-09-04 2015-11 1059-910X https://pubs.kist.re.kr/handle/201004/124853 The morphological and quantitative differences between arthritic fibroblast-like synoviocytes (FLS) and normal FLS were determined as an effective diagnostic tool for rheumatoid arthritis (RA), and confirmed using atomic force microscopy (AFM). Collagen-induced arthritic (CIA) mice and normal mice were prepared and FLS were isolated by enzymatic digestion from the synovial tissue of sacrificed mice at 5-week and 8-week pathogenesis periods. Analysis of cell morphology using AFM revealed that the surface roughness around the nucleus and around the branched cytoplasm was significantly higher in CIA FLS (P<0.05) than that in normal FLS. In addition, the roughness of two different sites on the arthritic FLS increased with an increase in the duration of pathogenesis. These results strongly suggest that AFM can be widely used as a diagnostic tool in cytopathology to detect the early signs of RA and various others diseases at the intercellular level. Microsc. Res. Tech. 78:982-988, 2015. (c) 2015 Wiley Periodicals, Inc. English WILEY EARLY RHEUMATOID-ARTHRITIS COLLAGEN-INDUCED ARTHRITIS NF-KAPPA-B SYNOVIAL FIBROBLASTS BONE EROSION CELLS EXPRESSION ULTRASONOGRAPHY INFLAMMATION JOINTS Detecting changes in arthritic fibroblast-like synoviocytes using atomic force microscopy Article 10.1002/jemt.22562 1 MICROSCOPY RESEARCH AND TECHNIQUE, v.78, no.11, pp.982 - 988 MICROSCOPY RESEARCH AND TECHNIQUE 78 11 982 988 scie scopus 000363726100005 2-s2.0-84945463983 Anatomy & Morphology Biology Microscopy Anatomy & Morphology Life Sciences & Biomedicine - Other Topics Microscopy Article EARLY RHEUMATOID-ARTHRITIS COLLAGEN-INDUCED ARTHRITIS NF-KAPPA-B SYNOVIAL FIBROBLASTS BONE EROSION CELLS EXPRESSION ULTRASONOGRAPHY INFLAMMATION JOINTS Atomic force microscopy fibroblast-like synoviocyte roughness of cellular surface morphology rheumatoid arthritis