Lee, Joon-Hyuk Shin, Hee-Sup Lee, Kwang-Hyung Chung, Sooyoung 2024-01-20T06:00:58Z 2024-01-20T06:00:58Z 2021-09-05 2015-10-29 2045-2322 https://pubs.kist.re.kr/handle/201004/124866 Two-photon microscopy of bulk-loaded functional dyes is an outstanding physiological technique that enables simultaneous functional mapping of hundreds of brain cells in vivo at single-cell resolution. However, precise targeting of a specific cortical location is not easy due to its fine dimensionality. To enable precise targeting, intrinsic-signal optical imaging is often additionally performed. However, the intrinsic-signal optical imaging is not only time-consuming but also ineffective in ensuring precision. Here, we propose an alternative method for precise targeting based on local field potential (LFP) recording, a conventional electrophysiological method. The heart of this method lies in use of the same glass pipette to record LFPs and to eject calcium dye. After confirming the target area by LFP using a glass pipette, the calcium dye is ejected from the same pipette without a time delay or spatial adjustment. As a result, the calcium dye is loaded into the same ensemble of brain cells from which the LFP was obtained. As a validation of the proposed LFP-based method, we targeted and successfully loaded calcium dye into layer 2/3 of a mouse barrel column. English NATURE PUBLISHING GROUP PRIMARY SOMATOSENSORY CORTEX MOUSE VISUAL-CORTEX RECEPTIVE-FIELDS RESPONSE PROPERTIES PYRAMIDAL CELLS SENSORY CORTEX SINGLE NEURONS REPRESENTATION ORIENTATION MICE LFP-guided targeting of a cortical barrel column for in vivo two-photon calcium imaging Article 10.1038/srep15905 1 SCIENTIFIC REPORTS, v.5 SCIENTIFIC REPORTS 5 scie scopus 000363628900001 2-s2.0-84946150394 Multidisciplinary Sciences Science & Technology - Other Topics Article PRIMARY SOMATOSENSORY CORTEX MOUSE VISUAL-CORTEX RECEPTIVE-FIELDS RESPONSE PROPERTIES PYRAMIDAL CELLS SENSORY CORTEX SINGLE NEURONS REPRESENTATION ORIENTATION MICE two-photon imaging calcium imaging targeting local field potentials in vivo mouse barrel cortex