Kim, Myungsuk Lim, Sue Ji Lee, Hee-Ju Nho, Chu Won 2024-01-20T06:01:07Z 2024-01-20T06:01:07Z 2021-09-04 2015-10-21 0021-8561 https://pubs.kist.re.kr/handle/201004/124874 Cassia tora seed is widely used due to its various biological properties including anticancer, antidiabetic, and anti-inflammatory effects. However, there has been no report of the effects of C. tora seed extract (CTE) on immunoglobulin E (IgE)mediated allergic responses. In this research, we demonstrated the effects of CTE and its active compound aurantio-obtusin on IgE-sensitized allergic reactions in mast cells and passive cutaneous anaphylaxis (PCA). CTE and aurantio-obtusin suppressed degranulation, histamine production, and reactive oxygen species generation and inhibited the production and mRNA expression of tumor necrosis factor-a and interleukin-4. CTE and aurantio-obtusin also suppressed the prostaglandin E-2 production and expression of cydooxygenase 2. Furthermore, CTE and aurantio-obtusin suppressed IgE-mediated Fc epsilon RI signaling such as phosphorylation of Syk, protein kinase C mu, phospholipase C gamma, and extracellular signal-regulated kinases. CTE and aurantio-obtusin blocked mast cell-dependent PCA in IgE-mediated mice. These results suggest that CTE and aurantio-obtusin are a beneficial treatment for allergy-related diseases. English AMER CHEMICAL SOC IN-VITRO ANTHRAQUINONE RBL-2H3 DEGRANULATION ACTIVATION RELEASE L. GENERATION OXIDANTS PATHWAY Cassia tora Seed Extract and Its Active Compound Aurantio-obtusin Inhibit Allergic Responses in IgE-Mediated Mast Cells and Anaphylactic Models Article 10.1021/acs.jafc.5b03836 1 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.63, no.41, pp.9037 - 9046 JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63 41 9037 9046 scie scopus 000363438700009 2-s2.0-84945246660 Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology Agriculture Chemistry Food Science & Technology Article IN-VITRO ANTHRAQUINONE RBL-2H3 DEGRANULATION ACTIVATION RELEASE L. GENERATION OXIDANTS PATHWAY aurantio-obtusin Cassia tora IgE-mediated allergic diseases RBL-2H3 mast cells