Kwon, Man Jae Yang, Jung-Seok Lee, Seunghak Lee, Giehyeon Ham, Baknoon Boyanov, Maxim I. Kemner, Kenneth M. O'Loughlin, Edward J. 2024-01-20T06:01:21Z 2024-01-20T06:01:21Z 2021-09-05 2015-10-15 0304-3894 https://pubs.kist.re.kr/handle/201004/124886 The effects of extreme geochemical conditions on microbial community composition were investigated for two distinct sets of sediment samples collected near weathered mine tailings. One set (SCH) showed extraordinary geochemical characteristics: As (6.7-11.5%), Pb (1.5-2.1%), Zn (0.1-0.2%), and pH (3.1-3.5). The other set (SCL) had As (0.3-1.2%), Pb (0.02-0.22%), and Zn (0.01-0.02%) at pH 2.5-3.1. The bacterial communities in SCL were clearly different from those in SCH, suggesting that extreme geochemical conditions affected microbial community distribution even on a small spatial scale. The clones identified in SCL were closely related to acidophilic bacteria in the taxa Acidobacterium (18%), Acidomicrobineae (14%), and Leptospirillum (10%). Most clones in SCH were closely related to Methylobacterium (79%) and Ralstonia (19%), both well-known metal-resistant bacteria. Although total As was extremely high, over 95% was in the form of scorodite (FeAsO4 center dot 2H(2)O). Acid-extractable As was only similar to 118 and similar to 14 mg kg(-1) in SCH and SCL, respectively, below the level known to be toxic to bacteria. Meanwhile, acid-extractable Pb and Zn in SCH were above toxic concentrations. Because As was present in an oxidized, stable form, release of Pb and/or Zn (or a combination of toxic metals in the sediment) from the sediment likely accounts for the differences in microbial community structure. The results also suggest that care should be taken when investigating mine tailings, because large differences in chemical/biological properties can occur over small spatial scales. (C) 2015 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV FRAGMENT-LENGTH-POLYMORPHISMS ARSENIC-RESISTANT BACTERIA RALSTONIA-METALLIDURANS LEAD RESISTANCE HEAVY-METALS SP NOV. MANGANESE SCORODITE ZINC ADSORPTION Geochemical characteristics and microbial community composition in toxic metal-rich sediments contaminated with Au-Ag mine tailings Article 10.1016/j.jhazmat.2015.04.035 1 JOURNAL OF HAZARDOUS MATERIALS, v.296, pp.147 - 157 JOURNAL OF HAZARDOUS MATERIALS 296 147 157 scie scopus 000356995500018 2-s2.0-84928253974 Engineering, Environmental Environmental Sciences Engineering Environmental Sciences & Ecology Article FRAGMENT-LENGTH-POLYMORPHISMS ARSENIC-RESISTANT BACTERIA RALSTONIA-METALLIDURANS LEAD RESISTANCE HEAVY-METALS SP NOV. MANGANESE SCORODITE ZINC ADSORPTION Mine tailings Toxic metals Microbial community Scorodite