Yang, Yoosoo Heo, Paul Kong, Byoungjae Park, Jun-Bum Jung, Young-Hun Shin, Jonghyeok Jeong, Cherlhyun Kweon, Dae-Hyuk 2024-01-20T06:01:44Z 2024-01-20T06:01:44Z 2021-09-05 2015-10-02 0006-291X https://pubs.kist.re.kr/handle/201004/124906 Soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) proteins generate energy required for membrane fusion. They form a parallelly aligned four-helix bundle called the SNARE complex, whose formation is initiated from the N terminus and proceeds toward the membraneproximal C terminus. Previously, we have shown that this zippering-like process can be controlled by several flavonoids that bind to the intermediate structures formed during the SNARE zippering. Here, our aim was to test whether the fluorescence resonance energy transfer signals that are observed during the inner leaflet mixing assay indeed represent the hemifused vesicles. We show that changes in vesicle size accompanying the merging of bilayers is a good measure of progression of the membrane fusion. Two merging vesicles with the same size D in diameter exhibited their hydrodynamic diameters 2D + d (d, intermembrane distance), 2D and root 2D as membrane fusion progressed from vesicle docking to hemifusion and full fusion, respectively. A dynamic light scattering assay of membrane fusion suggested that myricetin stopped membrane fusion at the hemifusion state, whereas delphinidin and cyanidin prevented the docking of the vesicles. These results are consistent with our previous findings in fluorescence resonance energy transfer assays. (C) 2015 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE SYNAPTIC EXOCYTOSIS COMPLEX Dynamic light scattering analysis of SNARE-driven membrane fusion and the effects of SNARE-binding flavonoids Article 10.1016/j.bbrc.2015.08.111 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.465, no.4, pp.864 - 870 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 465 4 864 870 scie scopus 000361782500033 2-s2.0-84941314743 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article SYNAPTIC EXOCYTOSIS COMPLEX SNARE Membrane fusion Dynamic light scattering FRET Flavonoid