Seong, Jihye 2024-01-20T06:02:45Z 2024-01-20T06:02:45Z 2021-09-05 2015-10 1738-2203 https://pubs.kist.re.kr/handle/201004/124960 Cells recognize and process various extracellular signals via transmembrane receptors and membrane-associated signaling molecules. Therefore, the precise regulation of these signaling events at the plasma membrane, which occur spatiotemporally, is necessary for proper cellular functions such as cell proliferation, migration, and survival. The plasma membrane may contain dynamic microdomains called lipid rafts, which are suggested to be crucial for the regulation of efficient and specific signaling pathways. However, because of the limitation in methodologies, the specific molecular mechanisms underlying dynamic signaling events at lipid rafts are largely unknown. This review discusses the traditional biochemical methods for the visualization of lipid rafts and the related signaling events at these microdomains. In addition, the review reports on fluorescence resonance energy transfer (FRET)-based molecular biosensors with lipid rafts targeting sequences as a powerful tool for live-cell imaging of spatiotemporal signaling events at lipid rafts. In particular, examples of dynamic lipid rafts signaling mechanisms visualized by FRET-based biosensors in live cells are covered in the last section. English KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY FOCAL ADHESION KINASE RICH MEMBRANE DOMAINS GPI-ANCHORED PROTEINS PLASMA-MEMBRANE MICRODOMAINS ACTIVATION SRC CHOLESTEROL CAMP ORGANIZATION Lipid rafts signaling detected by FRET-based molecular biosensors Article 10.1007/s13765-015-0082-2 1 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.58, no.5, pp.629 - 636 JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY 58 5 629 636 scie scopus kci ART002041596 000360374100001 2-s2.0-84940381934 Food Science & Technology Food Science & Technology Review FOCAL ADHESION KINASE RICH MEMBRANE DOMAINS GPI-ANCHORED PROTEINS PLASMA-MEMBRANE MICRODOMAINS ACTIVATION SRC CHOLESTEROL CAMP ORGANIZATION Acylation Biosensor Fluorescence resonance energy transfer Lipid rafts Live-cell imaging Prenylation Signaling