Park, Jong-Min Kim, Jo-Il Song, Hyan-Woo Noh, Joo-Yoon Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T06:03:48Z 2024-01-20T06:03:48Z 2021-09-05 2015-09-15 0956-5663 https://pubs.kist.re.kr/handle/201004/125015 This work presented a highly sensitive bacterial antibiotic susceptibility test through beta-lactamase assay using Parylene-matrix chip. beta-lactamases (EC 3.5.2.6) are an important family of enzymes that confer resistance to beta-lactam antibiotics by catalyzing the hydrolysis of these antibiotics. Here we present a highly sensitive assay to quantitate beta-lactamase-mediated hydrolysis of penicillin into penicilloic acid. Typically, MALDI-TOF mass spectrometry has been used to quantitate low molecular weight analytes and to discriminate them from noise peaks of matrix fragments that occur at low m/z ratios (m/z < 500). The beta-lactamase assay for the Escherichia coli antibiotic susceptibility test was carried out using Parylene-matrix chip and MALDI-TOF mass spectrometry. The Parylene-matrix chip was successfully used to quantitate penicillin (m/z: [PEN+H](+) =335.1 and [PEN+Na](+) =357.8) and penicilloic acid (m/z: [PA+H](+) =353.1) in a beta-lactamase assay with minimal interference of low molecular weight noise peaks. The beta-lactamase assay was carried out with an antibiotic-resistant E. coli strain and an antibiotic-susceptible E. coli strain, revealing that the minimum number of E. coli cells required to screen for antibiotic resistance was 1000 cells for the MALDI-TOF mass spectrometry/Parylene-matrix chip assay. (C) 2015 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY FLIGHT MASS-SPECTROMETRY ASSISTED LASER-DESORPTION AUTODISPLAYED Z-DOMAINS COLI OUTER-MEMBRANE MALDI-TOF-MS BETA-LACTAMASES QUANTITATIVE-ANALYSIS CARBON NANOTUBES SMALL MOLECULES SPR BIOSENSOR Highly sensitive bacterial susceptibility test against penicillin using parylene-matrix chip Article 10.1016/j.bios.2015.04.059 1 BIOSENSORS & BIOELECTRONICS, v.71, pp.306 - 312 BIOSENSORS & BIOELECTRONICS 71 306 312 scie scopus 000356646000045 2-s2.0-84928331263 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article FLIGHT MASS-SPECTROMETRY ASSISTED LASER-DESORPTION AUTODISPLAYED Z-DOMAINS COLI OUTER-MEMBRANE MALDI-TOF-MS BETA-LACTAMASES QUANTITATIVE-ANALYSIS CARBON NANOTUBES SMALL MOLECULES SPR BIOSENSOR MALDI-TOF mass spectrometry Parylene-matrix chip beta-lactamase Penicillin