Jeon, Hojeong Koo, Sangmo Reese, Willie Mae Loskill, Peter Grigoropoulos, Costas P. Healy, Kevin E. 2024-01-20T06:04:36Z 2024-01-20T06:04:36Z 2021-09-05 2015-09 1476-1122 https://pubs.kist.re.kr/handle/201004/125056 Although adhesive interactions between cells and nanostructured interfaces have been studied extensively(1-6), there is a paucity of data on how nanostructured interfaces repel cells by directing cell migration and cell-colony organization. Here, by using multiphoton ablation lithography(7) to pattern surfaces with nanoscale craters of various aspect ratios and pitches, we show that the surfaces altered the cells' focal-adhesion size and distribution, thus affecting cell morphology, migration and ultimately localization. We also show that nanocrater pitch can disrupt the formation of mature focal adhesions to favour the migration of cells towards higher-pitched regions, which present increased planar area for the formation of stable focal adhesions. Moreover, by designing surfaces with variable pitch but constant nanocrater dimensions, we were able to create circular and striped cellular patterns. Our surface-patterning approach, which does not involve chemical treatments and can be applied to various materials, represents a simple method to control cell behaviour on surfaces. English Nature Publishing Group Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces Article 10.1038/NMAT4342 1 Nature Materials, v.14, no.9, pp.918 - + Nature Materials 14 9 918 + N scie scopus 000360192000026 2-s2.0-84939776757 Chemistry, Physical Materials Science, Multidisciplinary Physics, Applied Physics, Condensed Matter Chemistry Materials Science Physics Article ADHESION NANOSCALE TOPOGRAPHY SUBSTRATE BINDING SURFACES FILMS cell migration cell repellent cell control nanopattern femtosecond laser nanocrater