Seo, Hong Seog Choi, Man Ho 2024-01-20T06:30:24Z 2024-01-20T06:30:24Z 2021-09-05 2015-09 0960-0760 https://pubs.kist.re.kr/handle/201004/125078 Despite the biochemical importance of cholesterol, its abnormal metabolism has serious cellular consequences that lead to endocrine disorders such as cardiovascular disease (CVD). Nevertheless, the impact of blood cholesterol as a CVD risk factor is still debated, and treatment with cholesterol-lowering drugs remains controversial, particularly in older patients. Although, the prevalence of CVD increases with age, the underlying mechanisms for this phenomenon are not well understood, and metabolic changes have not been confirmed as predisposing factors of atherogenesis. The quantification of circulating biomarkers for cholesterol homeostasis is therefore warranted, and reference values for cholesterol absorption and synthesis should be determined in order to establish CVD risk factors. The traditional lipid profile is often derived rather than directly measured and lacks a universal standard to interpret the results. In contrast, mass spectrometry-based cholesterol profiling can accurately measure free cholesterol as a biologically active component. This approach allows to detect alterations in various metabolic pathways that control cholesterol homeostasis, by quantitative analysis of cholesterol and its precursors/metabolites as well as dietary sterols. An overview of the mechanism of cholesterol homeostasis under different physiological conditions may help to identify predictive biomarkers of concomitant atherosclerosis and conventional CVD risk factors. (C) 2015 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD CORONARY-HEART-DISEASE SERUM-CHOLESTEROL ATHEROSCLEROTIC LESIONS PLASMA-MEMBRANE DIETARY-CHOLESTEROL ACYLTRANSFERASE MACROPHAGES BIOSYNTHESIS RECEPTOR PATHWAY Cholesterol homeostasis in cardiovascular disease and recent advances in measuring cholesterol signatures Article 10.1016/j.jsbmb.2015.04.014 1 JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.153, pp.72 - 79 JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 153 72 79 scie scopus 000362382700009 2-s2.0-84941261690 Biochemistry & Molecular Biology Endocrinology & Metabolism Biochemistry & Molecular Biology Endocrinology & Metabolism Article CORONARY-HEART-DISEASE SERUM-CHOLESTEROL ATHEROSCLEROTIC LESIONS PLASMA-MEMBRANE DIETARY-CHOLESTEROL ACYLTRANSFERASE MACROPHAGES BIOSYNTHESIS RECEPTOR PATHWAY Cholesterol Homeostasis Cardiovascular disease Atherogenesis Mass spectrometry