Lee, Kyung Min Choi, Okkyoung Kim, Ki-Yeon Woo, Han Min Kim, Yunje Han, Sung Ok Sang, Byoung-In Um, Youngsoon 2024-01-20T06:30:46Z 2024-01-20T06:30:46Z 2021-09-04 2015-09 0141-5492 https://pubs.kist.re.kr/handle/201004/125098 To produce butyric acid from red algae such as Gelidium amansii in which galactose is a main carbohydrate, microorganisms utilizing galactose and tolerating inhibitors in hydrolysis including levulinic acid and 5-hydroxymethylfurfural (HMF) are required. A newly isolated bacterium, Clostridium sp. S1 produced butyric acid not only from galactose as the sole carbon source but also from a mixture of galactose and glucose through simultaneous utilization. Notably, Clostridium sp. S1 produced butyric acid and a small amount of acetic acid with the butyrate:acetate ratio of 45.4:1 and it even converted acetate to butyric acid. Clostridium sp. S1 tolerated 0.5-2 g levulinic acid/l and recovered from HMF inhibition at 0.6-2.5 g/l, resulting in 85-92 % butyric acid concentration of the control culture. When acid-pretreated G. amansii hydrolysate was used, Clostridium sp. S1 produced 4.83 g butyric acid/l from 10 g galactose/l and 1 g glucose/l. Clostridium sp. S1 produces butyric acid from red algae due to its characteristics in sugar utilization and tolerance to inhibitors, demonstrating its advantage as a red algae-utilizing microorganism. English Kluwer Academic Publishers Butyric acid production from red algae by a newly isolated Clostridium sp S1 Article 10.1007/s10529-015-1869-2 1 Biotechnology Letters, v.37, no.9, pp.1837 - 1844 Biotechnology Letters 37 9 1837 1844 N scie scopus 000360432100014 2-s2.0-84940462018 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article GELIDIUM-AMANSII SACCHAROMYCES-CEREVISIAE FERMENTATION HYDROLYSATE BIOTRANSFORMATION TYROBUTYRICUM CELLOBIOSE HMF Biofuel Butyric acid fermentation Clostridium Galactose Gelidium amansii Red algae hydrolysate