Song, Mi-Kyung Lee, Hyo-Sun Ryu, Jae-Chun 2024-01-20T06:31:30Z 2024-01-20T06:31:30Z 2021-09-05 2015-08-06 0300-483X https://pubs.kist.re.kr/handle/201004/125132 We have shown that aldehydes impact the bronchial airway gene expression associated with inflammatory responses. In this study, we sought to determine whether microRNA (miRNA) plays a role in regulating the airway gene expression response to aldehyde exposure. We analyzed the whole genome miRNA and mRNA expression profiles of human alveolar epithelial cells exposed to 3 aldehydes (propanal, butanal, and pentanal) to identify aldehyde-sensitive miRNAs and to characterize the relationships between miRNAs and the expression of candidate cytokine-related genes, which are activated in response to inflammatory signals. Microarray analysis identified 15 miRNAs for propanal, 25 miRNAs for butanal, and 10 miRNAs for pentanal, which were differentially expressed in A549 human alveolar epithelial cells compared with vehicle control samples. Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA mRNA correlations. Gene ontology (GO) analysis of putative target genes (443 genes for propanal, 2166 genes for butanal, and 364 genes for pentanal) showed that the biological category "cytokine-cytokine receptor interaction" was prominently annotated. Moreover, we detected increased levels of interleukin (IL)-6 and IL-8 released in the 3 aldehyde exposure groups. Through an integrated analysis of the miRNA and mRNA expression profiles of aldehydes, we provide evidence that aldehyde can affect cytokine-induced toxicity signaling. Therefore, this study demonstrates the added value of an integrated miRNA mRNA approach for identifying molecular events altered by environmental pollutants in an in vitro human model. (C) 2015 Elsevier Ireland Ltd. All rights reserved. English ELSEVIER IRELAND LTD ALVEOLAR EPITHELIAL-CELLS VOLATILE ORGANIC-COMPOUNDS GENE-EXPRESSION AIRWAY INFLAMMATION MAMMALIAN MICRORNAS A549 CELLS RAT-LIVER EXPOSURE CANCER FORMALDEHYDE Integrated analysis of microRNA and mRNA expression profiles highlights aldehyde-induced inflammatory responses in cells relevant for lung toxicity Article 10.1016/j.tox.2015.06.007 1 TOXICOLOGY, v.334, pp.111 - 121 TOXICOLOGY 334 111 121 scie scopus 000358392800011 2-s2.0-84934914056 Pharmacology & Pharmacy Toxicology Pharmacology & Pharmacy Toxicology Article ALVEOLAR EPITHELIAL-CELLS VOLATILE ORGANIC-COMPOUNDS GENE-EXPRESSION AIRWAY INFLAMMATION MAMMALIAN MICRORNAS A549 CELLS RAT-LIVER EXPOSURE CANCER FORMALDEHYDE microRNA Target mRNAs Aldehydes Inflammation Cytokine Microarray Gene ontology (GO)