Jang, Mihue Kim, Jong Hwan Nam, Hae Yun Kwon, Ick Chan Ahn, Hyung Jun 2024-01-20T06:32:05Z 2024-01-20T06:32:05Z 2021-09-05 2015-08 2041-1723 https://pubs.kist.re.kr/handle/201004/125163 For therapeutic applications of siRNA, there are technical challenges with respect to targeted and systemic delivery. We here report a new siRNA carrier, RNAtr NPs, in a way that multiple tandem copies of RNA hairpins as a result of rolling circle transcription (RCT) can be readily adapted in tumour-targeted and systemic siRNA delivery. RNAtr NPs provide a means of condensing large amounts of multimeric RNA transcripts into the compact nanoparticles, especially without the aid of polycationic agents, and thus reduce the risk of immunogenicity and cytotoxicity by avoiding the use of synthetic polycationic reagents. This strategy allows the design of a platform technology for systemic delivery of siRNA to tumour sites, because RCT reaction, which enzymatically generates RNA polymers in multiple copy numbers at low cost, can lead to directly accessible routes to targeted and systemic delivery. Therefore, RNAtr NPs suggest great potentials as the siRNA therapeutics for cancer treatment. English NATURE PUBLISHING GROUP IN-VIVO RNA-INTERFERENCE DRUG-DELIVERY GENE NANOPARTICLES ACID THERAPEUTICS MICROSPONGES HEPATOCYTES EXPRESSION Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription Article 10.1038/ncomms8930 1 NATURE COMMUNICATIONS, v.6 NATURE COMMUNICATIONS 6 scie scopus 000360346000012 2-s2.0-84938767300 Multidisciplinary Sciences Science & Technology - Other Topics Article IN-VIVO RNA-INTERFERENCE DRUG-DELIVERY GENE NANOPARTICLES ACID THERAPEUTICS MICROSPONGES HEPATOCYTES EXPRESSION siRNA delivery carrier RNA nanoparticle cancer therapy